Unusual myogenic and melanocytic differentiation of soft tissue pPNETs: An immunohistochemical and molecular study of 3 cases

Marta Barisella, Paola Collini, Marta Orsenigo, Antonella Aiello, Carlo Terenzio Paties, Palma Dileo, Silvana Pilotti

Research output: Contribution to journalArticle

Abstract

All of the members of the peripheral primitive neuroectodermal tumor family (Ewing sarcomas, neuroectodermal tumors of bone, peripheral neuroepitheliomas, and Askin tumors) have similar morphologic and immunophenotypical features (ie, the proliferation of small and medium-sized round cells in a fibrous background showing strong and diffuse immunohistochemical positivity for CD99), and the common cytogenetic abnormality of a nonrandom translocation involving the EWS gene and one of several members of the erythroblastosis virus transforming sequence family of transcription factors. The combination of clinical information and morphologic/immunophenotypical characteristics is usually sufficient for a correct diagnosis, but there are rare cases in which an unusual predominant or multidirectional immunophenotypical differentiation makes diagnosis a challenge and requires the use of molecular cytogenetic or molecular techniques. We describe 3 such cases in which we employed fluorescence in-situ hybridization analysis to detect translocation involving the EWS gene and reverse transcription polymerase chain reaction followed by sequencing to detect the fusion transcript EWS-FLI1.

Original languageEnglish
JournalAmerican Journal of Surgical Pathology
DOIs
Publication statusAccepted/In press - May 20 2010

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Peripheral Primitive Neuroectodermal Tumors
Neuroectodermal Tumors
Ewing's Sarcoma
Cytogenetic Analysis
Fluorescence In Situ Hybridization
Chromosome Aberrations
Genes
Reverse Transcription
Transcription Factors
Viruses
Bone and Bones
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Surgery
  • Pathology and Forensic Medicine
  • Anatomy

Cite this

Unusual myogenic and melanocytic differentiation of soft tissue pPNETs : An immunohistochemical and molecular study of 3 cases. / Barisella, Marta; Collini, Paola; Orsenigo, Marta; Aiello, Antonella; Paties, Carlo Terenzio; Dileo, Palma; Pilotti, Silvana.

In: American Journal of Surgical Pathology, 20.05.2010.

Research output: Contribution to journalArticle

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