Up-regulation of nuclear PLCβ1 in myogenic differentiation

Irene Faenza, Alberto Bavelloni, Roberta Fiume, Giovanna Lattanzi, Nadir M. Maraldi, R. Stewart Gilmour, Alberto M. Martelli, Pann Ghill Suh, Anna Maria Billi, Lucio Cocco

Research output: Contribution to journalArticle

Abstract

Phospholipase Cβ1 (PLCβ1) signaling in both cell proliferation and differentiation has been largely investigated, but its role in myoblast differentiation is still unclear. The C2C12 myogenic cell line has been used in this study in order to find out the role of the two subtypes of PLCβ1, i.e., a and b in this process. C2C12 myoblast proliferate in response to mitogens and upon mitogen withdrawal differentiates into multinucleated myotubes. We found that differentiation of C2C12 skeletal muscle cells is characterized by a marked increase in the amount of nuclear PLCβ1 a and PLCβ1 b Indeed, treatment with insulin induces a dramatic rise of both PLCβ1 subtypes expression and activity, as determined by immunochemical and enzymatic assays. Immunofluorescence experiments with anti-PLCβ1 specific monoclonal antibody showed a low level of cytoplasmatic and nuclear staining during the initial 12 h of differentiation whilst a massive nuclear staining is appreciable in differentiating cells. The time course of PLCβ1 expression versus Troponin T expression clearly indicates that the increase in the amount of PLCβ1 takes place 24 h earlier than that of Troponin T. Moreover, the overexpression of the PLCβ1 M2b mutant, lacking the nuclear localization signal and entirely located in the cytoplasm, represses the formation of mature multinucleated myotube. Taken together these results suggest that nuclear PLCβ1 is a key player in myoblast differentiation, functioning as a positive regulator of this process.

Original languageEnglish
Pages (from-to)446-452
Number of pages7
JournalJournal of Cellular Physiology
Volume195
Issue number3
DOIs
Publication statusPublished - Jun 1 2003

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Up-Regulation
Myoblasts
Troponin T
Skeletal Muscle Fibers
Mitogens
Cells
phospholipase C1
Staining and Labeling
Nuclear Localization Signals
Cell proliferation
Enzyme Assays
Muscle Cells
Fluorescent Antibody Technique
Muscle
Cell Differentiation
Assays
Cytoplasm
Skeletal Muscle
Monoclonal Antibodies
Cell Proliferation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Up-regulation of nuclear PLCβ1 in myogenic differentiation. / Faenza, Irene; Bavelloni, Alberto; Fiume, Roberta; Lattanzi, Giovanna; Maraldi, Nadir M.; Gilmour, R. Stewart; Martelli, Alberto M.; Suh, Pann Ghill; Billi, Anna Maria; Cocco, Lucio.

In: Journal of Cellular Physiology, Vol. 195, No. 3, 01.06.2003, p. 446-452.

Research output: Contribution to journalArticle

Faenza, I, Bavelloni, A, Fiume, R, Lattanzi, G, Maraldi, NM, Gilmour, RS, Martelli, AM, Suh, PG, Billi, AM & Cocco, L 2003, 'Up-regulation of nuclear PLCβ1 in myogenic differentiation', Journal of Cellular Physiology, vol. 195, no. 3, pp. 446-452. https://doi.org/10.1002/jcp.10264
Faenza, Irene ; Bavelloni, Alberto ; Fiume, Roberta ; Lattanzi, Giovanna ; Maraldi, Nadir M. ; Gilmour, R. Stewart ; Martelli, Alberto M. ; Suh, Pann Ghill ; Billi, Anna Maria ; Cocco, Lucio. / Up-regulation of nuclear PLCβ1 in myogenic differentiation. In: Journal of Cellular Physiology. 2003 ; Vol. 195, No. 3. pp. 446-452.
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