Up-regulation of the inflammatory-reparative phenotype in human prostate carcinoma

Linda Ravenna, Patrizio Sale, Maura Di Vito, Andrea Russo, Luisa Salvatori, Marco Tafani, Emanuela Mari, Steno Sentinelli, Elisa Petrangeli, Michele Gallucci, Franco Di Silverio, Matteo A. Russo

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND. Recent studies have underlined the role of tumor cells in the endogenous synthesis of pro-inflammatory molecules.Wetested whether malignant progression in prostate cancer was associated with the activation of a phenotype typical of the innate immune system. METHODS. The expression of a set of molecules involved in tissue inflammation and repair was measured by real-time PCR and Western blot analysis in prostate samples in the absence or slight presence of a detectable leukocyte infiltrate. Whole tumor and non-tumor samples were analyzed in addition to laser-capture microdissected tumor and host epithelium. Receptor for advanced glycation end products, purine receptor, inducible enzymes cyclooxygenase-2 and nitric oxide synthase-2, pentraxin-3 and growth-survival factor receptors such as epithelial growth factor and estrogen α and β receptors were all studied. RESULTS. A global survey approach showed an up-regulation in tumor samples of all of the studied genes, with the exception of ERβ.Alaser-capture microdissection approach highlighted over-expression of pro-inflammatory molecules in each tumor sample examined. Nuclear translocation of nuclear factor-kB subunit p65 was observed in tumor tissues. CONCLUSIONS. These data support the evidence that molecules typical of the innate immune system, similar to that of activated leukocytes, are produced by prostate epithelial cells and that their expression is up-regulated in malignant cells. We suggest that the observed proinflammatory and repair process activation may represent an important molecular mechanism in the progression of prostate cancer.

Original languageEnglish
Pages (from-to)1245-1255
Number of pages11
JournalProstate
Volume69
Issue number11
DOIs
Publication statusPublished - Aug 1 2009

Keywords

  • Estrogen receptors
  • Inflammatory and repair process
  • P2X7R
  • Prostate carcinoma
  • RAGE

ASJC Scopus subject areas

  • Urology
  • Oncology
  • Medicine(all)

Fingerprint Dive into the research topics of 'Up-regulation of the inflammatory-reparative phenotype in human prostate carcinoma'. Together they form a unique fingerprint.

Cite this