Upregulation of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) by 15-lipoxygenase-modified LDL in endothelial cells

Angela Pirillo, Alice Reduzzi, Nicola Ferri, Hartmut Kuhn, Alberto Corsini, Alberico L. Catapano

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: Lectin-like oxidized LDL receptor-1 (LOX-1), the endothelial receptor for OxLDL, is believed to be responsible for a number of OxLDL-induced effects in the endothelium. Methods and results: In the present study we showed that LDL modified by 15-lipoxygenase (15LO-LDL), a form of minimally modified lipoprotein, beside its ability to induce pro-inflammatory responses such as oxidative stress and the expression of adhesion molecules, significantly increases LOX-1 expression in endothelial cells, both at transcriptional and at protein level. Such effect is likely to be mediated by p38 MAPK and NF-kB pathways. We then permanently overexpressed LOX-1 in an endothelial cell line and showed that 15LO-LDL were a ligand for LOX-1, and that the interaction LOX-1/15LO-LDL upregulated ICAM-1 surface expression. Conclusion: Altogether these results indicate minimally modified LDL as a new inducer for LOX-1 expression and as a new ligand for LOX-1.

Original languageEnglish
Pages (from-to)331-337
Number of pages7
JournalAtherosclerosis
Volume214
Issue number2
DOIs
Publication statusPublished - Feb 2011

Keywords

  • 15-Lipoxygenase
  • Endothelial cells
  • ICAM-1
  • LDL
  • LOX-1

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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