Upregulation of p75 tumor necrosis factor alpha receptor in Mycobacterium avium-infected mice: Evidence for a functional role

Angelo Corti, Lanfranco Fattorini, Ove Fredrik Thoresen, Maria Luisa Ricci, Anna Gallizia, Micaela Pelagi, Yongjun Li, Graziella Orefici

Research output: Contribution to journalArticle

Abstract

The bacterial growth and the production of tumor necrosis factor alpha (TNF-α) and TNF receptors (TNF-Rs) in the spleen and blood of BALB/c mice challenged with Mycobacterium avium complex (MAC) were monitored. Infection developed in two phases: the first, up to day 21, was associated with rapid MAC multiplication in the spleen and a drop in the mycobacteremia, and the second was associated with control of the infection in both compartments. In the spleen, TNF-α and TNF-RII mRNA levels peaked on day 21 and then slowly decreased; however, no increase in the level of TNF-RI mRNA was observed throughout these experiments. The level of circulating soluble TNF-RII (sTNF- RII) was transiently increased after day 21. In a model in which overproduction of bioactive TNF-α was triggered in response to a second infection with MAC, an increased production of sTNF-RII by cultured splenocytes was also observed. Administration of an antagonist anti-TNF-RII monoclonal antibody (MAb 6G1) to infected mice inhibited the bacterial growth in the spleen, suggesting that the TNF-RII and/or sTNF-RH was functionally involved in the mechanisms that control the infection. Overall, these observations suggest that upregulation of TNF-RII or sTNF-RII contributes to modulation of the TNF-α antibacterial activity in MAC infections.

Original languageEnglish
Pages (from-to)5762-5767
Number of pages6
JournalInfection and Immunity
Volume67
Issue number11
Publication statusPublished - Nov 1999

    Fingerprint

ASJC Scopus subject areas

  • Immunology

Cite this

Corti, A., Fattorini, L., Thoresen, O. F., Ricci, M. L., Gallizia, A., Pelagi, M., Li, Y., & Orefici, G. (1999). Upregulation of p75 tumor necrosis factor alpha receptor in Mycobacterium avium-infected mice: Evidence for a functional role. Infection and Immunity, 67(11), 5762-5767.