Uptake and recycling of pro-BDNF for transmitter-induced secretion by cortical astrocytes

Matteo Bergami, Spartaco Santi, Elena Formaggio, Cinzia Cagnoli, Claudia Verderio, Robert Blum, Benedikt Berninger, Michela Matteoli, Marco Canossa

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Activity-dependent secretion of brain-derived neurotrophic factor (BDNF) is thought to enhance synaptic plasticity, but the mechanisms controlling extracellular availability and clearance of secreted BDNF are poorly understood. We show that BDNF is secreted in its precursor form (pro-BDNF) and is then cleared from the extracellular space through rapid uptake by nearby astrocytes after θ-burst stimulation in layer II/III of cortical slices, a paradigm resulting in long-term potentiation of synaptic transmission. Internalization of pro-BDNF occurs via the formation of a complex with the pan-neurotrophin receptor p75 and subsequent clathrin-dependent endocytosis. Fluorescence-tagged pro-BDNF and real-time total internal reflection fluorescence microscopy in cultured astrocytes is used to monitor single endocytic vesicles in response to the neurotransmitter glutamate. We find that endocytosed pro-BDNF is routed into a fast recycling pathway for subsequent soluble NSF attachment protein receptor-dependent secretion. Thus, astrocytes contain an endocytic compartment competent for pro-BDNF recycling, suggesting a specialized form of bidirectional communication between neurons and glia.

Original languageEnglish
Pages (from-to)213-221
Number of pages9
JournalJournal of Cell Biology
Volume183
Issue number2
DOIs
Publication statusPublished - Oct 20 2008

Fingerprint

Brain-Derived Neurotrophic Factor
Recycling
Astrocytes
Endocytosis
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
SNARE Proteins
Nerve Growth Factor Receptor
Transport Vesicles
Clathrin
Neuronal Plasticity
Long-Term Potentiation
Extracellular Space
Fluorescence Microscopy
Synaptic Transmission
Neuroglia
Neurotransmitter Agents
Glutamic Acid
Fluorescence
Neurons

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

Uptake and recycling of pro-BDNF for transmitter-induced secretion by cortical astrocytes. / Bergami, Matteo; Santi, Spartaco; Formaggio, Elena; Cagnoli, Cinzia; Verderio, Claudia; Blum, Robert; Berninger, Benedikt; Matteoli, Michela; Canossa, Marco.

In: Journal of Cell Biology, Vol. 183, No. 2, 20.10.2008, p. 213-221.

Research output: Contribution to journalArticle

Bergami, M, Santi, S, Formaggio, E, Cagnoli, C, Verderio, C, Blum, R, Berninger, B, Matteoli, M & Canossa, M 2008, 'Uptake and recycling of pro-BDNF for transmitter-induced secretion by cortical astrocytes', Journal of Cell Biology, vol. 183, no. 2, pp. 213-221. https://doi.org/10.1083/jcb.200806137
Bergami, Matteo ; Santi, Spartaco ; Formaggio, Elena ; Cagnoli, Cinzia ; Verderio, Claudia ; Blum, Robert ; Berninger, Benedikt ; Matteoli, Michela ; Canossa, Marco. / Uptake and recycling of pro-BDNF for transmitter-induced secretion by cortical astrocytes. In: Journal of Cell Biology. 2008 ; Vol. 183, No. 2. pp. 213-221.
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