TY - JOUR
T1 - URB expression in human bone marrow stromal cells and during mouse development
AU - Liu, Yi
AU - Monticone, Massimiliano
AU - Tonachini, Laura
AU - Mastrogiacomo, Maddalena
AU - Marigo, Valeria
AU - Cancedda, Ranieri
AU - Castagnola, Patrizio
PY - 2004/9/17
Y1 - 2004/9/17
N2 - Seven genes preferentially expressed in undifferentiated human bone marrow stromal cells (BMSC) with respect to BMSC-derived osteoblasts were previously identified by differential display. Here we characterize the expression of one of these genes, URB, belonging to the sushi-repeat-containing protein superfamily. In culture, URB is expressed in both human primary and cloned BMSC, and is drastically downregulated during osteoblastic differentiation of these cells. Here we show that in mouse tissues a single 3.8 kb Urb transcript is detected and that the mouse Urb protein is secreted as a 150 kDa glycoprotein. During mouse development Urb RNA is barely detectable in 9 dpc embryos and increases at later stages. Both in situ hybridization and immunohistochemistry analysis show Urb expression in mouse embryos starting from 14 dpc mostly in cartilage. The temporal and spatial expression pattern of Urb suggests its role in mouse skeletogenesis.
AB - Seven genes preferentially expressed in undifferentiated human bone marrow stromal cells (BMSC) with respect to BMSC-derived osteoblasts were previously identified by differential display. Here we characterize the expression of one of these genes, URB, belonging to the sushi-repeat-containing protein superfamily. In culture, URB is expressed in both human primary and cloned BMSC, and is drastically downregulated during osteoblastic differentiation of these cells. Here we show that in mouse tissues a single 3.8 kb Urb transcript is detected and that the mouse Urb protein is secreted as a 150 kDa glycoprotein. During mouse development Urb RNA is barely detectable in 9 dpc embryos and increases at later stages. Both in situ hybridization and immunohistochemistry analysis show Urb expression in mouse embryos starting from 14 dpc mostly in cartilage. The temporal and spatial expression pattern of Urb suggests its role in mouse skeletogenesis.
KW - BMSC
KW - Cartilage
KW - Extracellular matrix
KW - Hypertrophic chondrocyte
KW - Skeletogenesis
KW - Sushi-repeat-containing protein
KW - Urb
UR - http://www.scopus.com/inward/record.url?scp=6044247626&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=6044247626&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2004.07.161
DO - 10.1016/j.bbrc.2004.07.161
M3 - Article
C2 - 15325258
AN - SCOPUS:6044247626
VL - 322
SP - 497
EP - 507
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -