Urea, creatinine, and glucose determined in plasma and whole blood by a differential pH technique.

M. Ripamonti, A. Mosca, E. Rovida, M. Luzzana, L. Luzi, F. Ceriotti, F. Cottini, L. Rossi-Bernardi

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Abstract

We report the conditions (buffer composition and enzyme activity) required for estimating three frequently determined analytes--urea, glucose, and creatinine--by use of an improved version of the differential pH apparatus previously described (Clin Chem 29: 80-85, 1983). For each analyte, we used only one specific enzyme, thus avoiding a chain of auxiliary and indicator reactions. The method requires about a minute for each determination in undiluted plasma or whole blood.

Original languageEnglish
Pages (from-to)556-559
Number of pages4
JournalClinical Chemistry
Volume30
Issue number4
Publication statusPublished - Apr 1984

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ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Ripamonti, M., Mosca, A., Rovida, E., Luzzana, M., Luzi, L., Ceriotti, F., Cottini, F., & Rossi-Bernardi, L. (1984). Urea, creatinine, and glucose determined in plasma and whole blood by a differential pH technique. Clinical Chemistry, 30(4), 556-559.