1. 1. Uridine phosphorylase (EC 18.104.22.168) from Escherichia coli B is an oligomeric protein composed of four identical subunits of 29,000 mol. wt. 2. 2. The enzyme has four half-cystine residues per subunit titrable only in denaturing condition. No disulphide linkages either inter- or intra-chain are present. The isolectic point is 5.25. 3. 3. The enzyme shows strict specificity toward uridine and 5-methyluridine and is inhibited by thymine, deoxycytidine and heavy metal ions.
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