TY - JOUR
T1 - Urinary and plasma metabolite differences detected by HPLC-ESI-QTOF-MS in systemic sclerosis patients
AU - PRECISESADS Clinical Consortium
AU - Fernández-Ochoa, Álvaro
AU - Quirantes-Piné, Rosa
AU - Borrás-Linares, Isabel
AU - Gemperline, David
AU - Alarcón Riquelme, Marta E.
AU - Beretta, Lorenzo
AU - Segura-Carretero, Antonio
PY - 2019/1/5
Y1 - 2019/1/5
N2 - Systemic Sclerosis (SSc) is a chronic autoimmune disease whose origin and pathogenesis are not yet well known. Recent studies are allowing a better definition of the disease. However, few studies have been performed based on metabolomics. In this way, this study aims to find altered metabolites in SSc patients in order to improve their diagnosis, prognosis and treatment. For that, 59 SSc patients and 28 healthy volunteers participated in this study. Urine and plasma samples were analysed by a fingerprinting metabolomic approach based on HPLC-ESI-QTOF-MS. We observed larger differences in urine than plasma metabolites. The main deregulated metabolic families in urine were acylcarnitines, acylglycines and metabolites derived from amino acids, specifically from proline, histidine and glutamine. These results indicate perturbations in fatty acid beta oxidation and amino acid pathways in scleroderma patients. On the other hand, the main plasma biomarker candidate was 2-arachidonoylglycerol, which is involved in the endocannabinoid system with potential implications in the induction and propagation of systemic sclerosis and autoimmunity.
AB - Systemic Sclerosis (SSc) is a chronic autoimmune disease whose origin and pathogenesis are not yet well known. Recent studies are allowing a better definition of the disease. However, few studies have been performed based on metabolomics. In this way, this study aims to find altered metabolites in SSc patients in order to improve their diagnosis, prognosis and treatment. For that, 59 SSc patients and 28 healthy volunteers participated in this study. Urine and plasma samples were analysed by a fingerprinting metabolomic approach based on HPLC-ESI-QTOF-MS. We observed larger differences in urine than plasma metabolites. The main deregulated metabolic families in urine were acylcarnitines, acylglycines and metabolites derived from amino acids, specifically from proline, histidine and glutamine. These results indicate perturbations in fatty acid beta oxidation and amino acid pathways in scleroderma patients. On the other hand, the main plasma biomarker candidate was 2-arachidonoylglycerol, which is involved in the endocannabinoid system with potential implications in the induction and propagation of systemic sclerosis and autoimmunity.
KW - 2-arachidonoylglycerol
KW - Acylcarnitines
KW - Biomarker
KW - HPLC-ESI-QTOF-MS
KW - Metabolomics
KW - Systemic sclerosis
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U2 - 10.1016/j.jpba.2018.09.021
DO - 10.1016/j.jpba.2018.09.021
M3 - Article
C2 - 30227356
AN - SCOPUS:85053393669
VL - 162
SP - 82
EP - 90
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
SN - 0731-7085
ER -