Urinary excretion and origin of 11-dehydro-2,3-dinorthromboxane B2 in man

C. Chiabrando, L. Rivoltella, E. Alberti, M. Bonollo, R. Djurup, R. Fanelli

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The in vivo biosynthesis of thromboxane B2 (TXB2) in man is currently evaluated by measuring urinary excretion of its major urinary metabolites, 11-dehydro-TXB2 and 2,3-dinor-TXB2. 11-Dehydro-2,3-dinor-TXB2, another prominent metabolite of exogenous TXB2 in man, has never been measured in human urine. We measured urinary 11-dehydro-2,3-dinor-TXB2 in parallel with 11-dehydro-TXB2 and 2,3-dinor-TXB2 by immunoaffinity extraction/gas chromatography-mass spectrometry in healthy non-smokers (n=12) and age-matched smokers (n=11). In non-smokers, urinary excretion of 11-dehydro-2,3-dinor-TXB2, 11-dehydro-TXB2 and 2,3-dinor-TXB2 was 29.7±11.1, 53.6±15.0 and 13.5±2.8 ng/h (mean±SD), respectively. In smokers, only urinary excretion of 2,3-dinor-TXB2 was significantly different (19.7±6.7 ng/h, p

Original languageEnglish
Pages (from-to)401-411
Number of pages11
JournalProstaglandins
Volume45
Issue number5
DOIs
Publication statusPublished - 1993

Fingerprint

Thromboxane B2
Metabolites
Biosynthesis
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Urine
2,3-dinor-thromboxane B2
11-dehydro-2,3-dinor-thromboxane B2
11-dehydro-thromboxane B2

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Urinary excretion and origin of 11-dehydro-2,3-dinorthromboxane B2 in man. / Chiabrando, C.; Rivoltella, L.; Alberti, E.; Bonollo, M.; Djurup, R.; Fanelli, R.

In: Prostaglandins, Vol. 45, No. 5, 1993, p. 401-411.

Research output: Contribution to journalArticle

Chiabrando, C, Rivoltella, L, Alberti, E, Bonollo, M, Djurup, R & Fanelli, R 1993, 'Urinary excretion and origin of 11-dehydro-2,3-dinorthromboxane B2 in man', Prostaglandins, vol. 45, no. 5, pp. 401-411. https://doi.org/10.1016/0090-6980(93)90117-P
Chiabrando, C. ; Rivoltella, L. ; Alberti, E. ; Bonollo, M. ; Djurup, R. ; Fanelli, R. / Urinary excretion and origin of 11-dehydro-2,3-dinorthromboxane B2 in man. In: Prostaglandins. 1993 ; Vol. 45, No. 5. pp. 401-411.
@article{2535809bdf844ac88394deee532e22cf,
title = "Urinary excretion and origin of 11-dehydro-2,3-dinorthromboxane B2 in man",
abstract = "The in vivo biosynthesis of thromboxane B2 (TXB2) in man is currently evaluated by measuring urinary excretion of its major urinary metabolites, 11-dehydro-TXB2 and 2,3-dinor-TXB2. 11-Dehydro-2,3-dinor-TXB2, another prominent metabolite of exogenous TXB2 in man, has never been measured in human urine. We measured urinary 11-dehydro-2,3-dinor-TXB2 in parallel with 11-dehydro-TXB2 and 2,3-dinor-TXB2 by immunoaffinity extraction/gas chromatography-mass spectrometry in healthy non-smokers (n=12) and age-matched smokers (n=11). In non-smokers, urinary excretion of 11-dehydro-2,3-dinor-TXB2, 11-dehydro-TXB2 and 2,3-dinor-TXB2 was 29.7±11.1, 53.6±15.0 and 13.5±2.8 ng/h (mean±SD), respectively. In smokers, only urinary excretion of 2,3-dinor-TXB2 was significantly different (19.7±6.7 ng/h, p",
author = "C. Chiabrando and L. Rivoltella and E. Alberti and M. Bonollo and R. Djurup and R. Fanelli",
year = "1993",
doi = "10.1016/0090-6980(93)90117-P",
language = "English",
volume = "45",
pages = "401--411",
journal = "Prostaglandins and Other Lipid Mediators",
issn = "1098-8823",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Urinary excretion and origin of 11-dehydro-2,3-dinorthromboxane B2 in man

AU - Chiabrando, C.

AU - Rivoltella, L.

AU - Alberti, E.

AU - Bonollo, M.

AU - Djurup, R.

AU - Fanelli, R.

PY - 1993

Y1 - 1993

N2 - The in vivo biosynthesis of thromboxane B2 (TXB2) in man is currently evaluated by measuring urinary excretion of its major urinary metabolites, 11-dehydro-TXB2 and 2,3-dinor-TXB2. 11-Dehydro-2,3-dinor-TXB2, another prominent metabolite of exogenous TXB2 in man, has never been measured in human urine. We measured urinary 11-dehydro-2,3-dinor-TXB2 in parallel with 11-dehydro-TXB2 and 2,3-dinor-TXB2 by immunoaffinity extraction/gas chromatography-mass spectrometry in healthy non-smokers (n=12) and age-matched smokers (n=11). In non-smokers, urinary excretion of 11-dehydro-2,3-dinor-TXB2, 11-dehydro-TXB2 and 2,3-dinor-TXB2 was 29.7±11.1, 53.6±15.0 and 13.5±2.8 ng/h (mean±SD), respectively. In smokers, only urinary excretion of 2,3-dinor-TXB2 was significantly different (19.7±6.7 ng/h, p

AB - The in vivo biosynthesis of thromboxane B2 (TXB2) in man is currently evaluated by measuring urinary excretion of its major urinary metabolites, 11-dehydro-TXB2 and 2,3-dinor-TXB2. 11-Dehydro-2,3-dinor-TXB2, another prominent metabolite of exogenous TXB2 in man, has never been measured in human urine. We measured urinary 11-dehydro-2,3-dinor-TXB2 in parallel with 11-dehydro-TXB2 and 2,3-dinor-TXB2 by immunoaffinity extraction/gas chromatography-mass spectrometry in healthy non-smokers (n=12) and age-matched smokers (n=11). In non-smokers, urinary excretion of 11-dehydro-2,3-dinor-TXB2, 11-dehydro-TXB2 and 2,3-dinor-TXB2 was 29.7±11.1, 53.6±15.0 and 13.5±2.8 ng/h (mean±SD), respectively. In smokers, only urinary excretion of 2,3-dinor-TXB2 was significantly different (19.7±6.7 ng/h, p

UR - http://www.scopus.com/inward/record.url?scp=0027193768&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027193768&partnerID=8YFLogxK

U2 - 10.1016/0090-6980(93)90117-P

DO - 10.1016/0090-6980(93)90117-P

M3 - Article

VL - 45

SP - 401

EP - 411

JO - Prostaglandins and Other Lipid Mediators

JF - Prostaglandins and Other Lipid Mediators

SN - 1098-8823

IS - 5

ER -