Urinary exosomal MicroRNAs in incipient diabetic nephropathy

Federica Barutta, Marinella Tricarico, Alessandro Corbelli, Laura Annaratone, Silvia Pinach, Serena Grimaldi, Graziella Bruno, Daniela Cimino, Daniela Taverna, Maria Chiara Deregibus, Maria Pia Rastaldi, Paolo Cavallo Perin, Gabriella Gruden

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

MicroRNAs (miRNAs), a class of small non-protein-encoding RNAs, regulate gene expression via suppression of target mRNAs. MiRNAs are present in body fluids in a remarkable stable form as packaged in microvesicles of endocytic origin, named exosomes. In the present study, we have assessed miRNA expression in urinary exosomes from type 1 diabetic patients with and without incipient diabetic nephropathy. Results showed that miR-130a and miR-145 were enriched, while miR-155 and miR-424 reduced in urinary exosomes from patients with microalbuminuria. Similarly, in an animal model of early experimental diabetic nephropathy, urinary exosomal miR-145 levels were increased and this was paralleled by miR-145 overexpression within the glomeruli. Exposure of cultured mesangial cells to high glucose increased miR-145 content in both mesangial cells and mesangial cells-derived exosomes, providing a potential mechanism for diabetes-induced miR-145 overexpression. In conclusion, urinary exosomal miRNA content is altered in type 1 diabetic patients with incipient diabetic nephropathy and miR-145 may represent a novel candidate biomarker/player in the complication.

Original languageEnglish
Article numbere73798
JournalPLoS One
Volume8
Issue number11
DOIs
Publication statusPublished - Nov 4 2013

Fingerprint

exosomes
Exosomes
diabetic nephropathy
Diabetic Nephropathies
MicroRNAs
microRNA
Mesangial Cells
Body fluids
Biomarkers
Medical problems
Gene expression
body fluids
Animals
Body Fluids
RNA
cultured cells
diabetes
Glucose
Cultured Cells
biomarkers

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Barutta, F., Tricarico, M., Corbelli, A., Annaratone, L., Pinach, S., Grimaldi, S., ... Gruden, G. (2013). Urinary exosomal MicroRNAs in incipient diabetic nephropathy. PLoS One, 8(11), [e73798]. https://doi.org/10.1371/journal.pone.0073798

Urinary exosomal MicroRNAs in incipient diabetic nephropathy. / Barutta, Federica; Tricarico, Marinella; Corbelli, Alessandro; Annaratone, Laura; Pinach, Silvia; Grimaldi, Serena; Bruno, Graziella; Cimino, Daniela; Taverna, Daniela; Deregibus, Maria Chiara; Rastaldi, Maria Pia; Perin, Paolo Cavallo; Gruden, Gabriella.

In: PLoS One, Vol. 8, No. 11, e73798, 04.11.2013.

Research output: Contribution to journalArticle

Barutta, F, Tricarico, M, Corbelli, A, Annaratone, L, Pinach, S, Grimaldi, S, Bruno, G, Cimino, D, Taverna, D, Deregibus, MC, Rastaldi, MP, Perin, PC & Gruden, G 2013, 'Urinary exosomal MicroRNAs in incipient diabetic nephropathy', PLoS One, vol. 8, no. 11, e73798. https://doi.org/10.1371/journal.pone.0073798
Barutta F, Tricarico M, Corbelli A, Annaratone L, Pinach S, Grimaldi S et al. Urinary exosomal MicroRNAs in incipient diabetic nephropathy. PLoS One. 2013 Nov 4;8(11). e73798. https://doi.org/10.1371/journal.pone.0073798
Barutta, Federica ; Tricarico, Marinella ; Corbelli, Alessandro ; Annaratone, Laura ; Pinach, Silvia ; Grimaldi, Serena ; Bruno, Graziella ; Cimino, Daniela ; Taverna, Daniela ; Deregibus, Maria Chiara ; Rastaldi, Maria Pia ; Perin, Paolo Cavallo ; Gruden, Gabriella. / Urinary exosomal MicroRNAs in incipient diabetic nephropathy. In: PLoS One. 2013 ; Vol. 8, No. 11.
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