Urinary hydroxylysine glycosides: Solid phase extraction and reversed phase chromatographic measurement

E. Casari, M. Murone, V. Grazioli

Research output: Contribution to journalArticlepeer-review

Abstract

A method is described for measurement of urinary hydroxylysine glycosides (galactosyl-hydroxylysine, or GHYL, and glucosyl-galactosyl-hydroxylysine, or GGHYL) by reversed phase chromatography after purification of the sample by solid phase extraction. Urinary levels of hydroxylysine glycosides has been correlated with collagen catabolism, and the urinary GGHYL: GHYL ratio is indicative of the metabolism of connective tissue. The GGHYL:GHYL ratio might, therefore, be a promising indicator of bone mass loss. The method described is analytically reliable and enables the measurement of a possible marker for diagnosis and follow-up of osteoporosis.

Original languageEnglish
Pages (from-to)144-146
Number of pages3
JournalChromatographia
Volume36
Issue number1
DOIs
Publication statusPublished - Dec 1993

Keywords

  • Collagen
  • Column liquid chromatography
  • Hydroxylysine glycosides
  • Osteoporosis
  • Solid phase extraction

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

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