Urinary signatures of renal cell carcinoma investigated by peptidomic approaches

Clizia Chinello, Marta Cazzaniga, Gabriele De Sio, Andrew James Smith, Erica Gianazza, Angelica Grasso, Francesco Rocco, Stefano Signorini, Marco Grasso, Silvano Bosari, Italo Zoppis, Mohammed Dakna, Yuri E M Van Der Burgt, Giancarlo Mauri, Fulvio Magni

Research output: Contribution to journalArticlepeer-review

Abstract

Renal Cell Carcinoma (RCC) is typically asymptomatic and surgery usually increases patient's lifespan only for early stage tumours. Moreover, solid renal masses cannot be confidently differentiated from RCC. Therefore, markers to distinguish malignant kidney tumours and for their detection are needed. Two different peptide signatures were obtained by a MALDITOF profiling approach based on urine pre-purification by C8 magnetic beads. One cluster of 12 signals could differentiate malignant tumours (n = 137) from benign renal masses and controls (n = 153) with sensitivity of 76% and specificity of 87% in the validation set. A second cluster of 12 signals distinguished clear cell RCC (n = 118) from controls (n = 137) with sensitivity and specificity values of 84% and 91%, respectively. Most of the peptide signals used in the two models were observed at higher abundance in patient urines and could be identified as fragments of proteins involved in tumour pathogenesis and progression. Among them: The Meprin 1a with a pro-Angiogenic activity, the Probable G-protein coupled receptor 162, belonging to the GPCRs family and known to be associated with several key functions in cancer, the Osteopontin that strongly correlates to tumour stages and invasiveness, the Phosphorylase b kinase regulatory subunit alpha and the SeCreted and TransMembrane protein 1.

Original languageEnglish
Article numbere106684
JournalPLoS One
Volume9
Issue number9
DOIs
Publication statusPublished - Sep 1 2014

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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