Urinary testosterone measurement by gas chromatography after solid-phase extraction and high-performance liquid chromatography

Elisabetta Venturelli, Antonia Manzari, Adalberto Cavalleri, Maurizio Benzo, Giorgio Secreto, Ettore Marubini

Research output: Contribution to journalArticlepeer-review

Abstract

A method was developed for the rapid determination of testosterone in urine. The procedure consists of solid-phase extraction (SPE) followed by high-performance liquid chromatographic (HPLC) clean-up before gas chromatographic determination. Recovery was evaluated by adding [3H]testosterone (104 cpm) to urine samples; the mean recovery of radioactivity after SPE and HPLC was 82%. Precision was estimated by repeated measurement of testosterone in four different urine samples; the coefficient of variation was 7.9% (95% confidence limits 6.1-11.4%). Accuracy was evaluated by standard addition and dilution assays; a linear relationship was found between the expected and observed values (r2 = 0.982). The method is rapid, effective and suitable for routine analysis.

Original languageEnglish
Pages (from-to)7-12
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume582
Issue number1-2
DOIs
Publication statusPublished - Nov 6 1992

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Fingerprint Dive into the research topics of 'Urinary testosterone measurement by gas chromatography after solid-phase extraction and high-performance liquid chromatography'. Together they form a unique fingerprint.

Cite this