Abstract
Urine proteome is a potential source of information in renal diseases, and it is considered a natural area of investigation for biomarkers. Technology developments have markedly increased the power analysis on urinary proteins, and it is time to confront methodologies and results of major studies on the topics. This is a first part of a series of reviews that will focus on the urine proteome as a site for detecting biomarkers of renal diseases; the theme of the first review concerns methodological aspects applied to normal urine. Main issues are techniques for urine pretreatment, separation of exo-somes, use of combinatorial peptide ligand libraries, mass spectrometry approaches, and analysis of data sets. Available studies show important differences, suggesting a major confounding effect of the technologies utilized for analysis. The objective is to obtain consensus about which approaches should be utilized for studying urine proteome in renal diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 41-48 |
| Number of pages | 8 |
| Journal | Biomarker Insights |
| Volume | 11 |
| DOIs | |
| Publication status | Published - Mar 16 2016 |
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Keywords
- Combinatorial peptide ligand libraries
- Mass spectrometry
- Urinary proteome
- Vesicles
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology
- Biochemistry, medical
Cite this
Urine proteome biomarkers in kidney diseases. I. Limits, perspectives, and first focus on normal urine. / Santucci, Laura; Bruschi, Maurizio; Candiano, Giovanni; Lugani, Francesca; Petretto, Andrea; Bonanni, Alice; Ghiggeri, Gian Marco.
In: Biomarker Insights, Vol. 11, 16.03.2016, p. 41-48.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Urine proteome biomarkers in kidney diseases. I. Limits, perspectives, and first focus on normal urine
AU - Santucci, Laura
AU - Bruschi, Maurizio
AU - Candiano, Giovanni
AU - Lugani, Francesca
AU - Petretto, Andrea
AU - Bonanni, Alice
AU - Ghiggeri, Gian Marco
PY - 2016/3/16
Y1 - 2016/3/16
N2 - Urine proteome is a potential source of information in renal diseases, and it is considered a natural area of investigation for biomarkers. Technology developments have markedly increased the power analysis on urinary proteins, and it is time to confront methodologies and results of major studies on the topics. This is a first part of a series of reviews that will focus on the urine proteome as a site for detecting biomarkers of renal diseases; the theme of the first review concerns methodological aspects applied to normal urine. Main issues are techniques for urine pretreatment, separation of exo-somes, use of combinatorial peptide ligand libraries, mass spectrometry approaches, and analysis of data sets. Available studies show important differences, suggesting a major confounding effect of the technologies utilized for analysis. The objective is to obtain consensus about which approaches should be utilized for studying urine proteome in renal diseases.
AB - Urine proteome is a potential source of information in renal diseases, and it is considered a natural area of investigation for biomarkers. Technology developments have markedly increased the power analysis on urinary proteins, and it is time to confront methodologies and results of major studies on the topics. This is a first part of a series of reviews that will focus on the urine proteome as a site for detecting biomarkers of renal diseases; the theme of the first review concerns methodological aspects applied to normal urine. Main issues are techniques for urine pretreatment, separation of exo-somes, use of combinatorial peptide ligand libraries, mass spectrometry approaches, and analysis of data sets. Available studies show important differences, suggesting a major confounding effect of the technologies utilized for analysis. The objective is to obtain consensus about which approaches should be utilized for studying urine proteome in renal diseases.
KW - Combinatorial peptide ligand libraries
KW - Mass spectrometry
KW - Urinary proteome
KW - Vesicles
UR - http://www.scopus.com/inward/record.url?scp=84961825584&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84961825584&partnerID=8YFLogxK
U2 - 10.4137/BMIMI.S26229.
DO - 10.4137/BMIMI.S26229.
M3 - Article
AN - SCOPUS:84961825584
VL - 11
SP - 41
EP - 48
JO - Biomarker Insights
JF - Biomarker Insights
SN - 1177-2719
ER -