TY - JOUR
T1 - Urine sediment analysis
T2 - Analytical and diagnostic performance of sediMAX® - A new automated microscopy image-based urine sediment analyser
AU - Zaman, Zahur
AU - Fogazzi, Giovanni Battista
AU - Garigali, Giuseppe
AU - Croci, Maria Daniela
AU - Bayer, Gabor
AU - Kránicz, Tunde
PY - 2010/2/2
Y1 - 2010/2/2
N2 - Background: We evaluated the analytical and diagnostic performance of sediMAX (77 Elektronika, Budapest, Hungary), a new automated microscopy image-based urine sediment analyser (which in some countries is also called Urised) in comparison with visual phase-contrast microscopy. Methods: Precision, linearity, carry-over and method comparison were carried out according to well-established guidelines. The diagnostic performance with respect to visual phase-contrast microscopy was evaluated with results from two centres (n1 = 910, n2 = 1233). Uncentrifuged urine samples were used in visual microscopy in centre 1, while urine sediments were used in centre 2. Results: Within-run precision for RBC was 17.8% and 6.7% at 18 × E6 RBC/L and 447 × E6 RBC/L respectively and for WBC it was 17% and 4.4% at 4 × E6 WBC/L and 258 × E6/L respectively. Between-run imprecision for RBC was 14.7% for 30 × E6/L and 7.2% for 283 × E6/L. For WBC it was 5.4% at 25 × E6/L and 3% at 166 × E6/L. In both studies areas under ROC curves (AUC) were 80-90% for RBC, WBC, squamous epithelial cells, yeast and calcium-oxalate crystals. For non-squamous epithelial cells and pathological and hyaline casts the AUC ranged 73-74%. There was no carry-over. Conclusions: The sediMAX is well able to count and identify RBC, WBC, squamous epithelial cells, yeast, bacteria and calcium-oxalate crystals. Recognition of pathological casts and non-squamous epithelial cells is adequate but needs to be improved.
AB - Background: We evaluated the analytical and diagnostic performance of sediMAX (77 Elektronika, Budapest, Hungary), a new automated microscopy image-based urine sediment analyser (which in some countries is also called Urised) in comparison with visual phase-contrast microscopy. Methods: Precision, linearity, carry-over and method comparison were carried out according to well-established guidelines. The diagnostic performance with respect to visual phase-contrast microscopy was evaluated with results from two centres (n1 = 910, n2 = 1233). Uncentrifuged urine samples were used in visual microscopy in centre 1, while urine sediments were used in centre 2. Results: Within-run precision for RBC was 17.8% and 6.7% at 18 × E6 RBC/L and 447 × E6 RBC/L respectively and for WBC it was 17% and 4.4% at 4 × E6 WBC/L and 258 × E6/L respectively. Between-run imprecision for RBC was 14.7% for 30 × E6/L and 7.2% for 283 × E6/L. For WBC it was 5.4% at 25 × E6/L and 3% at 166 × E6/L. In both studies areas under ROC curves (AUC) were 80-90% for RBC, WBC, squamous epithelial cells, yeast and calcium-oxalate crystals. For non-squamous epithelial cells and pathological and hyaline casts the AUC ranged 73-74%. There was no carry-over. Conclusions: The sediMAX is well able to count and identify RBC, WBC, squamous epithelial cells, yeast, bacteria and calcium-oxalate crystals. Recognition of pathological casts and non-squamous epithelial cells is adequate but needs to be improved.
KW - Automated urine analysis
KW - sediMAX/Urised
KW - Urine microscopy
KW - Urine particles
KW - Urine sediment analysis
UR - http://www.scopus.com/inward/record.url?scp=72649087075&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=72649087075&partnerID=8YFLogxK
U2 - 10.1016/j.cca.2009.10.018
DO - 10.1016/j.cca.2009.10.018
M3 - Article
C2 - 19861122
AN - SCOPUS:72649087075
VL - 411
SP - 147
EP - 154
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
IS - 3-4
ER -