Urotensin-II and its receptor (UT-R) are expressed in rat brain endothelial cells, and urotensin-II via UT-R stimulates angiogenesis in vivo and in vitro

Raffaella Spinazzi, Giovanna Albertin, Beatrice Nico, Diego Guidolin, Rosa Di Liddo, Gian Paolo Rossi, Domenico Ribatti, Gastone G. Nussdorfer

Research output: Contribution to journalArticle

Abstract

Urotensin-II (UII), along its receptor UT-R, is widely expressed in the cardiovascular system, where it exerts regulatory actions under both physiological and pathological conditions. Real-time PCR and immunocytochemistry demonstrated the expression of UII and UT-R as mRNA and protein in rat neuromicrovascular endothelial cells (NECs). UII did not affect the proliferation rate of cultured NECs, but exerted a strong angiogenic action in both an in vitro assay on Matrigel and an in vivo assay on chorioallantoic membrane. The angiogenic effect of UII was similar to that of FGF-2, and was abolished by the UT-R antagonist Palosuran. Collectively, our findings allow us to include UII in the group of cytokines (e.g. endothelin-1 and adrenomedullin), which are expressed in endothelial cells and exert a pro-angiogenic effect acting in an autocrine-paracrine manner.

Original languageEnglish
Pages (from-to)1107-1112
Number of pages6
JournalInternational Journal of Molecular Medicine
Volume18
Issue number6
Publication statusPublished - Dec 2006

Fingerprint

Endothelial Cells
Brain
Adrenomedullin
Chorioallantoic Membrane
Endothelin-1
Fibroblast Growth Factor 2
Cardiovascular System
Real-Time Polymerase Chain Reaction
Immunohistochemistry
In Vitro Techniques
urotensin II
Cytokines
Messenger RNA
Proteins

Keywords

  • Angiogenesis
  • Endothelial cells
  • Palosuran
  • Rat
  • Urotensin receptors
  • Urotensin-II

ASJC Scopus subject areas

  • Genetics

Cite this

Spinazzi, R., Albertin, G., Nico, B., Guidolin, D., Di Liddo, R., Rossi, G. P., ... Nussdorfer, G. G. (2006). Urotensin-II and its receptor (UT-R) are expressed in rat brain endothelial cells, and urotensin-II via UT-R stimulates angiogenesis in vivo and in vitro. International Journal of Molecular Medicine, 18(6), 1107-1112.

Urotensin-II and its receptor (UT-R) are expressed in rat brain endothelial cells, and urotensin-II via UT-R stimulates angiogenesis in vivo and in vitro. / Spinazzi, Raffaella; Albertin, Giovanna; Nico, Beatrice; Guidolin, Diego; Di Liddo, Rosa; Rossi, Gian Paolo; Ribatti, Domenico; Nussdorfer, Gastone G.

In: International Journal of Molecular Medicine, Vol. 18, No. 6, 12.2006, p. 1107-1112.

Research output: Contribution to journalArticle

Spinazzi, R, Albertin, G, Nico, B, Guidolin, D, Di Liddo, R, Rossi, GP, Ribatti, D & Nussdorfer, GG 2006, 'Urotensin-II and its receptor (UT-R) are expressed in rat brain endothelial cells, and urotensin-II via UT-R stimulates angiogenesis in vivo and in vitro', International Journal of Molecular Medicine, vol. 18, no. 6, pp. 1107-1112.
Spinazzi, Raffaella ; Albertin, Giovanna ; Nico, Beatrice ; Guidolin, Diego ; Di Liddo, Rosa ; Rossi, Gian Paolo ; Ribatti, Domenico ; Nussdorfer, Gastone G. / Urotensin-II and its receptor (UT-R) are expressed in rat brain endothelial cells, and urotensin-II via UT-R stimulates angiogenesis in vivo and in vitro. In: International Journal of Molecular Medicine. 2006 ; Vol. 18, No. 6. pp. 1107-1112.
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AU - Albertin, Giovanna

AU - Nico, Beatrice

AU - Guidolin, Diego

AU - Di Liddo, Rosa

AU - Rossi, Gian Paolo

AU - Ribatti, Domenico

AU - Nussdorfer, Gastone G.

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AB - Urotensin-II (UII), along its receptor UT-R, is widely expressed in the cardiovascular system, where it exerts regulatory actions under both physiological and pathological conditions. Real-time PCR and immunocytochemistry demonstrated the expression of UII and UT-R as mRNA and protein in rat neuromicrovascular endothelial cells (NECs). UII did not affect the proliferation rate of cultured NECs, but exerted a strong angiogenic action in both an in vitro assay on Matrigel and an in vivo assay on chorioallantoic membrane. The angiogenic effect of UII was similar to that of FGF-2, and was abolished by the UT-R antagonist Palosuran. Collectively, our findings allow us to include UII in the group of cytokines (e.g. endothelin-1 and adrenomedullin), which are expressed in endothelial cells and exert a pro-angiogenic effect acting in an autocrine-paracrine manner.

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KW - Endothelial cells

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