Urotensin-II-stimulated expression of pro-angiogenic factors in human vascular endothelial cells

Giovanna Albertin, Diego Guidolin, Elisa Sorato, Barbara Oselladore, Cinzia Tortorella, Domenico Ribatti

Research output: Contribution to journalArticle

Abstract

Urotensin-II (U-II) is an endogenous peptide recently characterized as a "nonclassic" pro-angiogenic cytokine. In fact, human vascular endothelial cells express the U-II receptor and exhibit a strong in vitro angiogenic response to the peptide, which was specifically triggered by the binding of U-II to its receptor and involved the activation of ERK1/2 and PI3K/Akt signaling pathways. Moreover, available studies, designed to investigate the pro-angiogenic effect quite shortly following U-II stimulation, suggested that the angiogenic action of the peptide was direct and not associated with an increased expression of vascular endothelial growth factor (VEGF) and/or its receptors. In the present study, the expression of three pro-angiogenic factors, namely VEGF, endothelin-1, and adrenomedullin, was studied in human umbilical vein endothelial cells (HUVEC) for longer times of U-II stimulation. RT-PCR and Western blot indicated that in HUVEC, exposed for at least 24. h to U-II, the expression of the three angiogenic molecules was significantly increased at both mRNA and protein level, opening the possibility that U-II, not only could exert a direct stimulation of an angiogenic phenotype in endothelial cells quite shortly following exposure to the peptide, but could also further enhance the process indirectly by inducing in the cells a delayed production of other pro-angiogenic factors. Interestingly, a preliminary analysis of the time course of the in vitro capillary-like pattern formation was consistent with this view, suggesting a two phase temporal dynamics of the process.

Original languageEnglish
Pages (from-to)16-22
Number of pages7
JournalRegulatory Peptides
Volume172
Issue number1-3
DOIs
Publication statusPublished - Dec 10 2011

Fingerprint

Angiogenesis Inducing Agents
Endothelial cells
Endothelial Cells
Peptides
Human Umbilical Vein Endothelial Cells
Vascular Endothelial Growth Factor A
Angiogenic Proteins
Adrenomedullin
Endothelin-1
urotensin II
Phosphatidylinositol 3-Kinases
Western Blotting
Chemical activation
Cytokines
Phenotype
Polymerase Chain Reaction
Messenger RNA
Molecules
Proteins

Keywords

  • Angiogenesis
  • Angiogenic factors
  • Human endothelial cells
  • Urotensin-II

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Albertin, G., Guidolin, D., Sorato, E., Oselladore, B., Tortorella, C., & Ribatti, D. (2011). Urotensin-II-stimulated expression of pro-angiogenic factors in human vascular endothelial cells. Regulatory Peptides, 172(1-3), 16-22. https://doi.org/10.1016/j.regpep.2011.08.001

Urotensin-II-stimulated expression of pro-angiogenic factors in human vascular endothelial cells. / Albertin, Giovanna; Guidolin, Diego; Sorato, Elisa; Oselladore, Barbara; Tortorella, Cinzia; Ribatti, Domenico.

In: Regulatory Peptides, Vol. 172, No. 1-3, 10.12.2011, p. 16-22.

Research output: Contribution to journalArticle

Albertin, G, Guidolin, D, Sorato, E, Oselladore, B, Tortorella, C & Ribatti, D 2011, 'Urotensin-II-stimulated expression of pro-angiogenic factors in human vascular endothelial cells', Regulatory Peptides, vol. 172, no. 1-3, pp. 16-22. https://doi.org/10.1016/j.regpep.2011.08.001
Albertin, Giovanna ; Guidolin, Diego ; Sorato, Elisa ; Oselladore, Barbara ; Tortorella, Cinzia ; Ribatti, Domenico. / Urotensin-II-stimulated expression of pro-angiogenic factors in human vascular endothelial cells. In: Regulatory Peptides. 2011 ; Vol. 172, No. 1-3. pp. 16-22.
@article{1216f528a5fe470eaca92ed5e9ebb3e7,
title = "Urotensin-II-stimulated expression of pro-angiogenic factors in human vascular endothelial cells",
abstract = "Urotensin-II (U-II) is an endogenous peptide recently characterized as a {"}nonclassic{"} pro-angiogenic cytokine. In fact, human vascular endothelial cells express the U-II receptor and exhibit a strong in vitro angiogenic response to the peptide, which was specifically triggered by the binding of U-II to its receptor and involved the activation of ERK1/2 and PI3K/Akt signaling pathways. Moreover, available studies, designed to investigate the pro-angiogenic effect quite shortly following U-II stimulation, suggested that the angiogenic action of the peptide was direct and not associated with an increased expression of vascular endothelial growth factor (VEGF) and/or its receptors. In the present study, the expression of three pro-angiogenic factors, namely VEGF, endothelin-1, and adrenomedullin, was studied in human umbilical vein endothelial cells (HUVEC) for longer times of U-II stimulation. RT-PCR and Western blot indicated that in HUVEC, exposed for at least 24. h to U-II, the expression of the three angiogenic molecules was significantly increased at both mRNA and protein level, opening the possibility that U-II, not only could exert a direct stimulation of an angiogenic phenotype in endothelial cells quite shortly following exposure to the peptide, but could also further enhance the process indirectly by inducing in the cells a delayed production of other pro-angiogenic factors. Interestingly, a preliminary analysis of the time course of the in vitro capillary-like pattern formation was consistent with this view, suggesting a two phase temporal dynamics of the process.",
keywords = "Angiogenesis, Angiogenic factors, Human endothelial cells, Urotensin-II",
author = "Giovanna Albertin and Diego Guidolin and Elisa Sorato and Barbara Oselladore and Cinzia Tortorella and Domenico Ribatti",
year = "2011",
month = "12",
day = "10",
doi = "10.1016/j.regpep.2011.08.001",
language = "English",
volume = "172",
pages = "16--22",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Urotensin-II-stimulated expression of pro-angiogenic factors in human vascular endothelial cells

AU - Albertin, Giovanna

AU - Guidolin, Diego

AU - Sorato, Elisa

AU - Oselladore, Barbara

AU - Tortorella, Cinzia

AU - Ribatti, Domenico

PY - 2011/12/10

Y1 - 2011/12/10

N2 - Urotensin-II (U-II) is an endogenous peptide recently characterized as a "nonclassic" pro-angiogenic cytokine. In fact, human vascular endothelial cells express the U-II receptor and exhibit a strong in vitro angiogenic response to the peptide, which was specifically triggered by the binding of U-II to its receptor and involved the activation of ERK1/2 and PI3K/Akt signaling pathways. Moreover, available studies, designed to investigate the pro-angiogenic effect quite shortly following U-II stimulation, suggested that the angiogenic action of the peptide was direct and not associated with an increased expression of vascular endothelial growth factor (VEGF) and/or its receptors. In the present study, the expression of three pro-angiogenic factors, namely VEGF, endothelin-1, and adrenomedullin, was studied in human umbilical vein endothelial cells (HUVEC) for longer times of U-II stimulation. RT-PCR and Western blot indicated that in HUVEC, exposed for at least 24. h to U-II, the expression of the three angiogenic molecules was significantly increased at both mRNA and protein level, opening the possibility that U-II, not only could exert a direct stimulation of an angiogenic phenotype in endothelial cells quite shortly following exposure to the peptide, but could also further enhance the process indirectly by inducing in the cells a delayed production of other pro-angiogenic factors. Interestingly, a preliminary analysis of the time course of the in vitro capillary-like pattern formation was consistent with this view, suggesting a two phase temporal dynamics of the process.

AB - Urotensin-II (U-II) is an endogenous peptide recently characterized as a "nonclassic" pro-angiogenic cytokine. In fact, human vascular endothelial cells express the U-II receptor and exhibit a strong in vitro angiogenic response to the peptide, which was specifically triggered by the binding of U-II to its receptor and involved the activation of ERK1/2 and PI3K/Akt signaling pathways. Moreover, available studies, designed to investigate the pro-angiogenic effect quite shortly following U-II stimulation, suggested that the angiogenic action of the peptide was direct and not associated with an increased expression of vascular endothelial growth factor (VEGF) and/or its receptors. In the present study, the expression of three pro-angiogenic factors, namely VEGF, endothelin-1, and adrenomedullin, was studied in human umbilical vein endothelial cells (HUVEC) for longer times of U-II stimulation. RT-PCR and Western blot indicated that in HUVEC, exposed for at least 24. h to U-II, the expression of the three angiogenic molecules was significantly increased at both mRNA and protein level, opening the possibility that U-II, not only could exert a direct stimulation of an angiogenic phenotype in endothelial cells quite shortly following exposure to the peptide, but could also further enhance the process indirectly by inducing in the cells a delayed production of other pro-angiogenic factors. Interestingly, a preliminary analysis of the time course of the in vitro capillary-like pattern formation was consistent with this view, suggesting a two phase temporal dynamics of the process.

KW - Angiogenesis

KW - Angiogenic factors

KW - Human endothelial cells

KW - Urotensin-II

UR - http://www.scopus.com/inward/record.url?scp=80053571192&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053571192&partnerID=8YFLogxK

U2 - 10.1016/j.regpep.2011.08.001

DO - 10.1016/j.regpep.2011.08.001

M3 - Article

C2 - 21871928

AN - SCOPUS:80053571192

VL - 172

SP - 16

EP - 22

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 1-3

ER -