Ursodeoxycholate promotes protein phosphorylation in the cytosol of rat hepatocytes

Ferdinando Capuano, Michele Barone, Nicola D'Eri, Elisabetta Russo, Davide Varone, Antonio Francavilla, Sergio Papa

Research output: Contribution to journalArticle

Abstract

A study is presented of the effect of the bile salt ursodeoxycholate (UDC) on protein phosphorylation by [γ-32P]ATP in the cytosol from rat hepatocytes. Gel electrophoresis and corresponding autoradiograms of cytosolic proteins show that UDC promotes phosphorylation of at least eight different protein bands. Four of them (the 36, 60, 64 and 76 kDa) are phosphorylated by Ca2+ and phospholipid-dependent protein kinase (PKC); three (the 31, 51 and 71 kDa) are phosphorylated by cAMP-dependent protein kinase (PKA) and one protein band, with molecular weight of 34 kDa, apparently contains substrates of both PKC and PKA. Data are reported indicating that UDC can directly affect the intrinsic activity of protein kinases.

Original languageEnglish
Pages (from-to)329-337
Number of pages9
JournalBiochemistry and Molecular Biology International
Volume41
Issue number2
Publication statusPublished - Feb 1997

Fingerprint

Phosphorylation
Cytosol
Rats
Hepatocytes
Protein Kinases
Proteins
Cyclic AMP-Dependent Protein Kinases
Electrophoresis
Bile Acids and Salts
Phospholipids
Adenosine Triphosphate
Molecular Weight
Gels
Molecular weight
Substrates

Keywords

  • Bile salt
  • Ca and phospholipid-dependent protein kinase
  • cAMP-dependent protein kinase
  • Protein phosphorylation
  • Rat hepatocytes

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Capuano, F., Barone, M., D'Eri, N., Russo, E., Varone, D., Francavilla, A., & Papa, S. (1997). Ursodeoxycholate promotes protein phosphorylation in the cytosol of rat hepatocytes. Biochemistry and Molecular Biology International, 41(2), 329-337.

Ursodeoxycholate promotes protein phosphorylation in the cytosol of rat hepatocytes. / Capuano, Ferdinando; Barone, Michele; D'Eri, Nicola; Russo, Elisabetta; Varone, Davide; Francavilla, Antonio; Papa, Sergio.

In: Biochemistry and Molecular Biology International, Vol. 41, No. 2, 02.1997, p. 329-337.

Research output: Contribution to journalArticle

Capuano, F, Barone, M, D'Eri, N, Russo, E, Varone, D, Francavilla, A & Papa, S 1997, 'Ursodeoxycholate promotes protein phosphorylation in the cytosol of rat hepatocytes', Biochemistry and Molecular Biology International, vol. 41, no. 2, pp. 329-337.
Capuano F, Barone M, D'Eri N, Russo E, Varone D, Francavilla A et al. Ursodeoxycholate promotes protein phosphorylation in the cytosol of rat hepatocytes. Biochemistry and Molecular Biology International. 1997 Feb;41(2):329-337.
Capuano, Ferdinando ; Barone, Michele ; D'Eri, Nicola ; Russo, Elisabetta ; Varone, Davide ; Francavilla, Antonio ; Papa, Sergio. / Ursodeoxycholate promotes protein phosphorylation in the cytosol of rat hepatocytes. In: Biochemistry and Molecular Biology International. 1997 ; Vol. 41, No. 2. pp. 329-337.
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