Use of a BJAB-derived cell line for isolation of human herpesvirus 8

Paola Gasperini, Massimo Barbierato, Canio Martinelli, Paolo Rigotti, Francesco Marchini, Giulia Masserizzi, Francesco Leoncini, Luigi Chieco-Bianchi, Thomas F. Schulz, Maria Luisa Calabrò

Research output: Contribution to journalArticle

Abstract

Establishment of latently infected cell lines from primary effusion lymphomas (PEL) presently is the most efficient system for the propagation of clinical strains of human herpesvirus 8 (HHV-8) in culture. Here we describe a new approach to culture productively replicating HHV-8 from patient samples. A BJAB-derived B-cell line, BBF, was found to retain HHV-8 longer, to support the latent and lytic replication programs, and to produce transmissible virus. Supernatants from n-butyrate-treated peripheral blood mononuclear cells of 24 HHV-8-seropositive renal transplant recipients were used to infect BBF cells, and replicating virus was detected in cultures from 11 patients. Moreover, BBF cells infected with saliva strains showed a highly productive profile regardless of the initial viral load, which confirms that infectious HHV-8 can be present in saliva and also suggests that saliva strains may exhibit a high tropism for B lymphocytes. In conclusion, we established an in vitro system that efficiently detects HHV-8 in samples with low viral loads and that produces infectious progeny. BBF cells can be used to propagate HHV-8 from different biological samples as well as to clarify important issues related to virus-cell interactions in a context distinct from endothelial and PEL-derived cell lines.

Original languageEnglish
Pages (from-to)2866-2875
Number of pages10
JournalJournal of Clinical Microbiology
Volume43
Issue number6
DOIs
Publication statusPublished - Jun 2005

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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    Gasperini, P., Barbierato, M., Martinelli, C., Rigotti, P., Marchini, F., Masserizzi, G., Leoncini, F., Chieco-Bianchi, L., Schulz, T. F., & Calabrò, M. L. (2005). Use of a BJAB-derived cell line for isolation of human herpesvirus 8. Journal of Clinical Microbiology, 43(6), 2866-2875. https://doi.org/10.1128/JCM.43.6.2866-2875.2005