Use of a gelatin cryogel as biomaterial scaffold in the differentiation process of human bone marrow stromal cells

Lorenzo Fassina, Enrica Saino, Livia Visai, Maria Antonietta Avanzini, Maria Gabriella Cusella De Angelis, Francesco Benazzo, Sandra Van Vlierberghe, Peter Dubruel, Giovanni Magenes

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biomaterials have been widely used in reconstructive bone surgery to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, gelatin cryogel scaffolds are promising new biomaterials owing to their biocompatibility; in addition, the in vitro modification of biomaterials with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering. In this study we have followed a biomimetic strategy where differentiated human bone marrow stromal cells built their extracellular matrix onto gelatin cryogel scaffolds. In comparison with control conditions without differentiation medium, the use of a differentiation medium increased, in vitro, the coating of gelatin cryogel with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The differentiation medium aimed at obtaining a better in vitro modification of gelatin cryogel in terms of cell colonization and coating with osteogenic signals, like bone matrix proteins. The modified biomaterial could be used, in clinical applications, as an implant for bone repair.

Original languageEnglish
Title of host publication2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10
Pages247-250
Number of pages4
DOIs
Publication statusPublished - 2010
Event2010 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10 - Buenos Aires, Argentina
Duration: Aug 31 2010Sep 4 2010

Other

Other2010 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10
CountryArgentina
CityBuenos Aires
Period8/31/109/4/10

ASJC Scopus subject areas

  • Biomedical Engineering

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