Use of a gelatin cryogel as biomaterial scaffold in the differentiation process of human bone marrow stromal cells.

Lorenzo Fassina, Enrica Saino, Livia Visai, Maria Antonietta Avanzini, Maria Gabriella Cusella De Angelis, Francesco Benazzo, Sandra Van Vlierberghe, Peter Dubruel, Giovanni Magenes

Research output: Contribution to journalArticle

Abstract

Biomaterials have been widely used in reconstructive bone surgery to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, gelatin cryogel scaffolds are promising new biomaterials owing to their biocompatibility; in addition, the in vitro modification of biomaterials with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering. In this study we have followed a biomimetic strategy where differentiated human bone marrow stromal cells built their extracellular matrix onto gelatin cryogel scaffolds. In comparison with control conditions without differentiation medium, the use of a differentiation medium increased, in vitro, the coating of gelatin cryogel with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The differentiation medium aimed at obtaining a better in vitro modification of gelatin cryogel in terms of cell colonization and coating with osteogenic signals, like bone matrix proteins. The modified biomaterial could be used, in clinical applications, as an implant for bone repair.

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Cryogels
Biocompatible Materials
Gelatin
Mesenchymal Stromal Cells
Biomaterials
Scaffolds
Bone
Bone and Bones
Reconstructive Surgical Procedures
Collagen
Decorin
Biomimetics
Bone Matrix
Osteopontin
Collagen Type III
Osteocalcin
Proteins
Coatings
Tissue Engineering
Tissue regeneration

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Biomedical Engineering
  • Health Informatics

Cite this

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title = "Use of a gelatin cryogel as biomaterial scaffold in the differentiation process of human bone marrow stromal cells.",
abstract = "Biomaterials have been widely used in reconstructive bone surgery to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, gelatin cryogel scaffolds are promising new biomaterials owing to their biocompatibility; in addition, the in vitro modification of biomaterials with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering. In this study we have followed a biomimetic strategy where differentiated human bone marrow stromal cells built their extracellular matrix onto gelatin cryogel scaffolds. In comparison with control conditions without differentiation medium, the use of a differentiation medium increased, in vitro, the coating of gelatin cryogel with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The differentiation medium aimed at obtaining a better in vitro modification of gelatin cryogel in terms of cell colonization and coating with osteogenic signals, like bone matrix proteins. The modified biomaterial could be used, in clinical applications, as an implant for bone repair.",
author = "Lorenzo Fassina and Enrica Saino and Livia Visai and Avanzini, {Maria Antonietta} and {Cusella De Angelis}, {Maria Gabriella} and Francesco Benazzo and {Van Vlierberghe}, Sandra and Peter Dubruel and Giovanni Magenes",
year = "2010",
language = "English",
pages = "247--250",
journal = "Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference",
issn = "1557-170X",
publisher = "Institute of Electrical and Electronics Engineers Inc.",

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TY - JOUR

T1 - Use of a gelatin cryogel as biomaterial scaffold in the differentiation process of human bone marrow stromal cells.

AU - Fassina, Lorenzo

AU - Saino, Enrica

AU - Visai, Livia

AU - Avanzini, Maria Antonietta

AU - Cusella De Angelis, Maria Gabriella

AU - Benazzo, Francesco

AU - Van Vlierberghe, Sandra

AU - Dubruel, Peter

AU - Magenes, Giovanni

PY - 2010

Y1 - 2010

N2 - Biomaterials have been widely used in reconstructive bone surgery to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, gelatin cryogel scaffolds are promising new biomaterials owing to their biocompatibility; in addition, the in vitro modification of biomaterials with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering. In this study we have followed a biomimetic strategy where differentiated human bone marrow stromal cells built their extracellular matrix onto gelatin cryogel scaffolds. In comparison with control conditions without differentiation medium, the use of a differentiation medium increased, in vitro, the coating of gelatin cryogel with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The differentiation medium aimed at obtaining a better in vitro modification of gelatin cryogel in terms of cell colonization and coating with osteogenic signals, like bone matrix proteins. The modified biomaterial could be used, in clinical applications, as an implant for bone repair.

AB - Biomaterials have been widely used in reconstructive bone surgery to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, gelatin cryogel scaffolds are promising new biomaterials owing to their biocompatibility; in addition, the in vitro modification of biomaterials with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering. In this study we have followed a biomimetic strategy where differentiated human bone marrow stromal cells built their extracellular matrix onto gelatin cryogel scaffolds. In comparison with control conditions without differentiation medium, the use of a differentiation medium increased, in vitro, the coating of gelatin cryogel with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The differentiation medium aimed at obtaining a better in vitro modification of gelatin cryogel in terms of cell colonization and coating with osteogenic signals, like bone matrix proteins. The modified biomaterial could be used, in clinical applications, as an implant for bone repair.

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