Use of a T-cell interferon-γ release assay for the diagnosis of tuberculous pleurisy

M. Losi, A. Bossink, L. Codecasa, C. Jafari, M. Ernst, S. Thijsen, D. Cirillo, M. Ferrarese, U. Greinert, L. M. Fabbri, L. Richeldi, C. Lange

Research output: Contribution to journalArticle

Abstract

The diagnosis of pleural tuberculosis (plTB) by the analysis of pleural effusions (PEs) with standard diagnostic tools is difficult. In routine clinical practice, the present authors evaluated the performance of a commercially available Mycobacterium tuberculosis (MTB)-specific enzyme-linked immunospot assay on peripheral blood mononuclear cells (PBMCs) and pleural effusion mononuclear cells (PEMCs) in patients with suspect plTB. The T-SPOT.TB test (Oxford Immunotec Ltd, Abingdon, UK) was performed on PBMCs and PEMCs in 20 patients with a clinical and radiological suspect of plTB and in 21 control subjects with a diagnosis of PE of nontuberculous origin at four centres participating in the European Tuberculosis Network. In total, 18 (90%) out of 20 patients with plTB tested T-SPOT.TB-positive on PBMCs and 19 (95%) out of 20 on PEMCs. Among controls, T-SPOT.TB was positive in seven out of 21 (33%) patients when performed on PBMCs (these patients were assumed to be latently infected with MTB) and five (23%) out of 21 when performed on PEMCs. Sensitivity and specificity of T-SPOT.TB for the diagnosis of active plTB when performed on PEMCs were 95 and 76%, respectively. Enumerating Mycobacterium tuberculosis-specific T-cells in pleural effusion mononuclear cells by ELISPOT is feasible in routine clinical practice and may be useful for a rapid and accurate diagnosis of pleural tuberculosis. Copyright

Original languageEnglish
Pages (from-to)1173-1179
Number of pages7
JournalEuropean Respiratory Journal
Volume30
Issue number6
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Pleural Tuberculosis
Pleural Effusion
Interferons
T-Lymphocytes
Blood Cells
Mycobacterium tuberculosis
Enzyme-Linked Immunospot Assay
Tuberculosis
Sensitivity and Specificity

Keywords

  • Culture filtrate protein-10
  • Early secretory antigenic target-6
  • Pleurisy
  • T-cell interferon-γ release assay
  • Tuberculosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Losi, M., Bossink, A., Codecasa, L., Jafari, C., Ernst, M., Thijsen, S., ... Lange, C. (2007). Use of a T-cell interferon-γ release assay for the diagnosis of tuberculous pleurisy. European Respiratory Journal, 30(6), 1173-1179. https://doi.org/10.1183/09031936.00067307

Use of a T-cell interferon-γ release assay for the diagnosis of tuberculous pleurisy. / Losi, M.; Bossink, A.; Codecasa, L.; Jafari, C.; Ernst, M.; Thijsen, S.; Cirillo, D.; Ferrarese, M.; Greinert, U.; Fabbri, L. M.; Richeldi, L.; Lange, C.

In: European Respiratory Journal, Vol. 30, No. 6, 12.2007, p. 1173-1179.

Research output: Contribution to journalArticle

Losi, M, Bossink, A, Codecasa, L, Jafari, C, Ernst, M, Thijsen, S, Cirillo, D, Ferrarese, M, Greinert, U, Fabbri, LM, Richeldi, L & Lange, C 2007, 'Use of a T-cell interferon-γ release assay for the diagnosis of tuberculous pleurisy', European Respiratory Journal, vol. 30, no. 6, pp. 1173-1179. https://doi.org/10.1183/09031936.00067307
Losi, M. ; Bossink, A. ; Codecasa, L. ; Jafari, C. ; Ernst, M. ; Thijsen, S. ; Cirillo, D. ; Ferrarese, M. ; Greinert, U. ; Fabbri, L. M. ; Richeldi, L. ; Lange, C. / Use of a T-cell interferon-γ release assay for the diagnosis of tuberculous pleurisy. In: European Respiratory Journal. 2007 ; Vol. 30, No. 6. pp. 1173-1179.
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AU - Cirillo, D.

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