Use of adoptive transfer of T-cell-antigen-receptor-transgenic T cells for the study of T-cell activation in vivo

Kathryn A. Pape, Elizabeth R. Kearney, Alexander Khoruts, Anna Mondino, Rebecca Merica, Zong Ming Chen, Elizabeth Ingulli, Jennifer White, Julia G. Johnson, Marc K. Jenkins

Research output: Contribution to journalArticle

Abstract

Adoptive transfer of TCR-transgenic T cells uniformly expressing an identifiable TCR of known peptide/MHC specificity can be used to monitor the in vivo behavior of antigen-specific T cells. We have used this system to show that naive T cells are initially activated within the T-cell zones of secondary lymphoid tissue to proliferate in a B7-dependent manner. If adjuvants or inflammatory cytokines are present during this period, enhanced numbers of T cells accumulate, migrate into B-cell-rich follicles, and acquire the capacity to produce IFN-γ and help B cells produce IgG2a. If inflammation is absent, most of the initially activated antigen-specific T cells disappear without entering the follicles, and the survivors are poor producers of IL-2 and IFN-γ. Our results indicate that inflammatory mediators play a key role in regulating the anatomic location, clonal expansion, survival and lymphokine production potential of antigen-stimulated T cells in vivo.

Original languageEnglish
Pages (from-to)67-78
Number of pages12
JournalImmunological Reviews
Volume156
Publication statusPublished - 1997

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Use of adoptive transfer of T-cell-antigen-receptor-transgenic T cells for the study of T-cell activation in vivo'. Together they form a unique fingerprint.

  • Cite this

    Pape, K. A., Kearney, E. R., Khoruts, A., Mondino, A., Merica, R., Chen, Z. M., Ingulli, E., White, J., Johnson, J. G., & Jenkins, M. K. (1997). Use of adoptive transfer of T-cell-antigen-receptor-transgenic T cells for the study of T-cell activation in vivo. Immunological Reviews, 156, 67-78.