Use of commercial cards for freeing DNA from mycobacterial strains

Enrico Tortoli; Serena Baruzzo; Stefania Lauria; Alessandro Mariottini

Use of commercial cards for freeing DNA from mycobacterial strains

Enrico Tortoli, Serena Baruzzo, Stefania Lauria, Alessandro Mariottini

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

A new commercial system for rapid extraction of DNA, consisting of a card on which a drop of bacterial suspension is spotted, was evaluated with 43 mycobacterial strains. Once dried, a small disk of the seeded area was directly transferred in the amplification mixture. All the samples produced good quality amplification products which were satisfactorily sequenced.

Original language	English
Pages (from-to)	151-153
Number of pages	3
Journal	New Microbiologica
Volume	31
Issue number	1
Publication status	Published - Jan 2008

Keywords

DNA extraction
Genetic sequencing
Mycobacteria
PCR

ASJC Scopus subject areas

Microbiology (medical)
Microbiology

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title = "Use of commercial cards for freeing DNA from mycobacterial strains",

abstract = "A new commercial system for rapid extraction of DNA, consisting of a card on which a drop of bacterial suspension is spotted, was evaluated with 43 mycobacterial strains. Once dried, a small disk of the seeded area was directly transferred in the amplification mixture. All the samples produced good quality amplification products which were satisfactorily sequenced.",

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AU - Mariottini, Alessandro

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