Abstract
A new commercial system for rapid extraction of DNA, consisting of a card on which a drop of bacterial suspension is spotted, was evaluated with 43 mycobacterial strains. Once dried, a small disk of the seeded area was directly transferred in the amplification mixture. All the samples produced good quality amplification products which were satisfactorily sequenced.
| Original language | English |
|---|---|
| Pages (from-to) | 151-153 |
| Number of pages | 3 |
| Journal | New Microbiologica |
| Volume | 31 |
| Issue number | 1 |
| Publication status | Published - Jan 2008 |
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Keywords
- DNA extraction
- Genetic sequencing
- Mycobacteria
- PCR
ASJC Scopus subject areas
- Microbiology (medical)
- Microbiology
Cite this
Use of commercial cards for freeing DNA from mycobacterial strains. / Tortoli, Enrico; Baruzzo, Serena; Lauria, Stefania; Mariottini, Alessandro.
In: New Microbiologica, Vol. 31, No. 1, 01.2008, p. 151-153.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Use of commercial cards for freeing DNA from mycobacterial strains
AU - Tortoli, Enrico
AU - Baruzzo, Serena
AU - Lauria, Stefania
AU - Mariottini, Alessandro
PY - 2008/1
Y1 - 2008/1
N2 - A new commercial system for rapid extraction of DNA, consisting of a card on which a drop of bacterial suspension is spotted, was evaluated with 43 mycobacterial strains. Once dried, a small disk of the seeded area was directly transferred in the amplification mixture. All the samples produced good quality amplification products which were satisfactorily sequenced.
AB - A new commercial system for rapid extraction of DNA, consisting of a card on which a drop of bacterial suspension is spotted, was evaluated with 43 mycobacterial strains. Once dried, a small disk of the seeded area was directly transferred in the amplification mixture. All the samples produced good quality amplification products which were satisfactorily sequenced.
KW - DNA extraction
KW - Genetic sequencing
KW - Mycobacteria
KW - PCR
UR - http://www.scopus.com/inward/record.url?scp=40949166242&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=40949166242&partnerID=8YFLogxK
M3 - Article
C2 - 18437856
AN - SCOPUS:40949166242
VL - 31
SP - 151
EP - 153
JO - New Microbiologica
JF - New Microbiologica
SN - 1121-7138
IS - 1
ER -