Use of DNA microarrays to monitor host response to virus and virus-derived gene therapy vectors

Stefania Piersanti, Yuri Martina, Gioia Cherubini, Daniele Avitabile, Isabella Saggio

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Given the biological complexity of viral infections, the variability of the host response, and the safety concerns related to viral-mediated gene transfer, recent studies have made use of DNA mircoarrays to integrate multi-layered experimental approaches aimed at completely clarifying virus-host interactions. Particular attention has been given to those viruses that are implicated in clinical use and/or in life-threatening diseases. Examples of such use can be divided into three main categories, including: (i) the use of microarrays to study viral expression; (ii) the use of microarrays to analyze the host response to viral infection; and (iii) the use of microarrays to characterize the host response to viral vector-mediated transduction. Significant information on virus- and viral vector-host interactions can be obtained with the microarray approach, including the recognition of master pathways of virally-induced responses, the identification of new target genes for specific viruses, and indications on the molecular toxicity of specific gene transfer vectors currently used for gene therapy trials (in particular, adeno-associated viruses and adenovirus-derived vectors). We predict that the development of accessible repositories containing most of the DNA microarray data on viral infections will certainly help to elucidate the puzzling pictures of different viral infections. This will be crucially important for the correct handling of viral diseases and the intelligent amelioration of viral vectors for gene therapy.

Original languageEnglish
Pages (from-to)345-356
Number of pages12
JournalAmerican Journal of PharmacoGenomics
Volume4
Issue number6
DOIs
Publication statusPublished - 2004

Fingerprint

Virus Diseases
Oligonucleotide Array Sequence Analysis
Genetic Therapy
Viruses
Viral Genes
Dependovirus
Microarray Analysis
Adenoviridae
Genes
Safety
DNA

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine
  • Genetics

Cite this

Use of DNA microarrays to monitor host response to virus and virus-derived gene therapy vectors. / Piersanti, Stefania; Martina, Yuri; Cherubini, Gioia; Avitabile, Daniele; Saggio, Isabella.

In: American Journal of PharmacoGenomics, Vol. 4, No. 6, 2004, p. 345-356.

Research output: Contribution to journalArticle

Piersanti, Stefania ; Martina, Yuri ; Cherubini, Gioia ; Avitabile, Daniele ; Saggio, Isabella. / Use of DNA microarrays to monitor host response to virus and virus-derived gene therapy vectors. In: American Journal of PharmacoGenomics. 2004 ; Vol. 4, No. 6. pp. 345-356.
@article{33d211b5f2144acdb433b906777f5e64,
title = "Use of DNA microarrays to monitor host response to virus and virus-derived gene therapy vectors",
abstract = "Given the biological complexity of viral infections, the variability of the host response, and the safety concerns related to viral-mediated gene transfer, recent studies have made use of DNA mircoarrays to integrate multi-layered experimental approaches aimed at completely clarifying virus-host interactions. Particular attention has been given to those viruses that are implicated in clinical use and/or in life-threatening diseases. Examples of such use can be divided into three main categories, including: (i) the use of microarrays to study viral expression; (ii) the use of microarrays to analyze the host response to viral infection; and (iii) the use of microarrays to characterize the host response to viral vector-mediated transduction. Significant information on virus- and viral vector-host interactions can be obtained with the microarray approach, including the recognition of master pathways of virally-induced responses, the identification of new target genes for specific viruses, and indications on the molecular toxicity of specific gene transfer vectors currently used for gene therapy trials (in particular, adeno-associated viruses and adenovirus-derived vectors). We predict that the development of accessible repositories containing most of the DNA microarray data on viral infections will certainly help to elucidate the puzzling pictures of different viral infections. This will be crucially important for the correct handling of viral diseases and the intelligent amelioration of viral vectors for gene therapy.",
author = "Stefania Piersanti and Yuri Martina and Gioia Cherubini and Daniele Avitabile and Isabella Saggio",
year = "2004",
doi = "10.2165/00129785-200404060-00002",
language = "English",
volume = "4",
pages = "345--356",
journal = "American Journal of PharmacoGenomics",
issn = "1175-2203",
publisher = "Adis International Ltd",
number = "6",

}

TY - JOUR

T1 - Use of DNA microarrays to monitor host response to virus and virus-derived gene therapy vectors

AU - Piersanti, Stefania

AU - Martina, Yuri

AU - Cherubini, Gioia

AU - Avitabile, Daniele

AU - Saggio, Isabella

PY - 2004

Y1 - 2004

N2 - Given the biological complexity of viral infections, the variability of the host response, and the safety concerns related to viral-mediated gene transfer, recent studies have made use of DNA mircoarrays to integrate multi-layered experimental approaches aimed at completely clarifying virus-host interactions. Particular attention has been given to those viruses that are implicated in clinical use and/or in life-threatening diseases. Examples of such use can be divided into three main categories, including: (i) the use of microarrays to study viral expression; (ii) the use of microarrays to analyze the host response to viral infection; and (iii) the use of microarrays to characterize the host response to viral vector-mediated transduction. Significant information on virus- and viral vector-host interactions can be obtained with the microarray approach, including the recognition of master pathways of virally-induced responses, the identification of new target genes for specific viruses, and indications on the molecular toxicity of specific gene transfer vectors currently used for gene therapy trials (in particular, adeno-associated viruses and adenovirus-derived vectors). We predict that the development of accessible repositories containing most of the DNA microarray data on viral infections will certainly help to elucidate the puzzling pictures of different viral infections. This will be crucially important for the correct handling of viral diseases and the intelligent amelioration of viral vectors for gene therapy.

AB - Given the biological complexity of viral infections, the variability of the host response, and the safety concerns related to viral-mediated gene transfer, recent studies have made use of DNA mircoarrays to integrate multi-layered experimental approaches aimed at completely clarifying virus-host interactions. Particular attention has been given to those viruses that are implicated in clinical use and/or in life-threatening diseases. Examples of such use can be divided into three main categories, including: (i) the use of microarrays to study viral expression; (ii) the use of microarrays to analyze the host response to viral infection; and (iii) the use of microarrays to characterize the host response to viral vector-mediated transduction. Significant information on virus- and viral vector-host interactions can be obtained with the microarray approach, including the recognition of master pathways of virally-induced responses, the identification of new target genes for specific viruses, and indications on the molecular toxicity of specific gene transfer vectors currently used for gene therapy trials (in particular, adeno-associated viruses and adenovirus-derived vectors). We predict that the development of accessible repositories containing most of the DNA microarray data on viral infections will certainly help to elucidate the puzzling pictures of different viral infections. This will be crucially important for the correct handling of viral diseases and the intelligent amelioration of viral vectors for gene therapy.

UR - http://www.scopus.com/inward/record.url?scp=11144241331&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11144241331&partnerID=8YFLogxK

U2 - 10.2165/00129785-200404060-00002

DO - 10.2165/00129785-200404060-00002

M3 - Article

C2 - 15651896

AN - SCOPUS:11144241331

VL - 4

SP - 345

EP - 356

JO - American Journal of PharmacoGenomics

JF - American Journal of PharmacoGenomics

SN - 1175-2203

IS - 6

ER -