Use of FTA® classic cards for epigenetic analysis of sperm DNA

Olga Serra, Raffaele Frazzi, Alessio Perotti, Lorenzo Barusi, Annamaria Buschini

Research output: Contribution to journalArticle

Abstract

FTA® technologies provide the most reliable method for DNA extraction. Although FTA technologies have been widely used for genetic analysis, there is no literature on their use for epigenetic analysis yet. We present for the first time, a simple method for quantitative methylation assessment based on sperm cells stored on Whatman FTA classic cards. Specifically, elution of seminal DNA from FTA classic cards was successfully tested with an elution buffer and an incubation step in a thermocycler. The eluted DNA was bisulfite converted, amplified by PCR, and a region of interest was pyrosequenced.

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalBioTechniques
Volume64
Issue number2
DOIs
Publication statusPublished - Feb 1 2018

Keywords

  • DNA methylation
  • Infertility
  • MEST
  • Pyrosequencing
  • Sperm cell

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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