Use of fusion proteins and procaryotic display systems for delivery of HIV-1 antigens: development of novel vaccines for HIV-1 infection.

Piergiuseppe De Berardinis, Rossella Sartorius, Antonella Caivano, Dina Mascolo, Gonzalo J. Domingo, Giovanna Del Pozzo, Muriel Gaubin, Richard N. Perham, Dominique Piatier-Tonneau, John Guardiola

Research output: Contribution to journalArticle

Abstract

Two non-pathogenic scaffolds (represented by the filamentous bacteriophage fd and the dihydrolipoyl acetyltransferase E2 protein of the Bacillus stearothermophilus pyruvate dehydrogenase (PDH) complex) able to deliver human immunodeficiency virus (HIV)-1 antigenic determinants, were designed in our laboratories and investigated in controlled assay conditions. Based on a modification of the phage display technology, we developed an innovative concept for a safe and inexpensive vaccine in which conserved antigenic determinants of HIV-1 reverse transcriptase (RTase) were inserted into the N-terminal region of the major pVIII coat protein of bacteriophagefd virions. Analogously, we developed another antigen delivery system based on the E2 component from the PDH complex and capable of displaying large intact proteins on the surface of an icosahedral lattice. Our data show that both of these systems can deliver B and T epitopes to their respective presentation compartments in target cells and trigger a humoral response as well as a potent helper and cytolytic response in vitro and in vivo.

Original languageEnglish
Pages (from-to)441-446
Number of pages6
JournalCurrent HIV Research
Volume1
Issue number4
Publication statusPublished - Oct 2003

ASJC Scopus subject areas

  • Infectious Diseases

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    De Berardinis, P., Sartorius, R., Caivano, A., Mascolo, D., Domingo, G. J., Del Pozzo, G., Gaubin, M., Perham, R. N., Piatier-Tonneau, D., & Guardiola, J. (2003). Use of fusion proteins and procaryotic display systems for delivery of HIV-1 antigens: development of novel vaccines for HIV-1 infection. Current HIV Research, 1(4), 441-446.