Use of macroporous polypropylene filter to allow identification of bacteria by PCR in human fecal samples

Aldo Cavallini, Maria Notarnicola, Pasquale Berloco, Antonio Lippolis, Alfredo Di Leo

Research output: Contribution to journalArticlepeer-review

Abstract

The detection of pathogenic bacteria directly in human fecal specimens by PCR, requires removal of PCR-inhibitory substances. To investigate whether five different macroporous filters (polypropylene, nylon, polyester, polyethylene, fluorocarbon) could retain polysaccharides, major PCR inhibitors, an in vitro model and human fecal samples were used. The in vitro model consisted of Xanthum gum solutions (3 mg/ml PBS), a bacterial polysaccharide, to which Helicobacter pylori cells were added. Fecal samples from healthy volunteers were spiked with H. pylori and Mycobacterium paratuberculosis cells. Polysaccharide concentrations were significantly reduced only by the polypropylene but not by the other filters. Accordingly, both Xanthum gum solutions and spiked fecal specimens became PCR positive only after filtration with the polypropylene filter. We conclude that this filter can be used to prepare a bacterial DNA template suitable for PCR analysis from human feces. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)265-270
Number of pages6
JournalJournal of Microbiological Methods
Volume39
Issue number3
DOIs
Publication statusPublished - Feb 2000

Keywords

  • Feces
  • PCR inhibitors
  • Polypropylene filter
  • Polysaccharides

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

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