Use of real-time polymerase chain reaction to differentiate between pathogenic entamoeba histolytica and the nonpathogenic entamoeba dispar in Ecuador

Ángel Guevara, Yosselin Vicuña, Denisse Costales, Sandra Vivero, Mariella Anselmi, Zeno Bisoffi, Fabio Formenti

Research output: Contribution to journalArticlepeer-review

Abstract

Microscopic examination of stool samples has been considered to be the “gold standard” for diagnosis of intestinal parasites. Recently, polymerase chain reaction (PCR) has been approved by the World Health Organization as the method of choice for the diagnosis of Entamoeba histolytica. Of the 106 stool samples collected from the Esmeraldas and Pichincha provinces of Ecuador, all (100%) were positive for E. histolytica/Entamoeba dispar by light microscopy, whereas using real-time PCR (RT-PCR) DNA amplification, 74 (69.8%) were positive for E. dispar and only three (2.8%) were positive for E. histolytica. Some 29 (27.4%) samples were negative for the presence of either E. histolytica or E. dispar, this may be due the presence of Entamoeba mosksvskii, which is morphologically identical to E. histolytica/ E. dispar and not specifically targeted by the RT-PCR used. These results indicate the necessity of reevaluating the epidemiology of amebiasis in Ecuador as the prominent species found are nonpathogenic.

Original languageEnglish
Pages (from-to)81-82
Number of pages2
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume100
Issue number1
DOIs
Publication statusPublished - Jan 1 2019

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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