TY - JOUR
T1 - Use of synthetic peptides to establish anti-human acetylcholine receceptor CD4 + cell lines from myasthenia gravis patients
AU - Protti, Maria Pia
AU - Manfredi, Angelo A.
AU - Straub, Christi
AU - Wu, Xiaodong
AU - Howard, James F.
AU - Conti-Tronconi, Bianca M.
PY - 1990/3/1
Y1 - 1990/3/1
N2 - Acetylcholine receptor-(AcChR) specific T cell lines were propagated from the PBL of six myasthenia gravis (MG) patients by the use of a pool of synthetic peptides (α-pool) corresponding to the complete sequence of the α-subunit of the human AcChR. All the lines had CD4+ phenotype and strongly recognized the α-pool. Four lines crossreacted with native Torpedo AcChR. Five lines showed, at certain stages of their propagation, some degree of reactivity to autologous or DR-matched APC. One of the CD4+ T lines was challenged with each one of the peptides present in the α-pool. Several peptides, corresponding to the sequence segments 48-67, 101-120, 304-322, 320-337, and 419-437 of the human α-subunit were recognized, indicating that different epitopes and multiple T cell clones are involved in the recognition of the autoantigen in MG. Human AcChR-specific CD4+ T cell lines will be useful to identify the repertoire of epitopes recognized by the autoreactive Th cells in MG, to investigate the TCR genes utilized by autoreactive Th cells and to develop specific immunosuppressive treatments using anti-T cell vaccination.
AB - Acetylcholine receptor-(AcChR) specific T cell lines were propagated from the PBL of six myasthenia gravis (MG) patients by the use of a pool of synthetic peptides (α-pool) corresponding to the complete sequence of the α-subunit of the human AcChR. All the lines had CD4+ phenotype and strongly recognized the α-pool. Four lines crossreacted with native Torpedo AcChR. Five lines showed, at certain stages of their propagation, some degree of reactivity to autologous or DR-matched APC. One of the CD4+ T lines was challenged with each one of the peptides present in the α-pool. Several peptides, corresponding to the sequence segments 48-67, 101-120, 304-322, 320-337, and 419-437 of the human α-subunit were recognized, indicating that different epitopes and multiple T cell clones are involved in the recognition of the autoantigen in MG. Human AcChR-specific CD4+ T cell lines will be useful to identify the repertoire of epitopes recognized by the autoreactive Th cells in MG, to investigate the TCR genes utilized by autoreactive Th cells and to develop specific immunosuppressive treatments using anti-T cell vaccination.
UR - http://www.scopus.com/inward/record.url?scp=0025309796&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025309796&partnerID=8YFLogxK
M3 - Article
C2 - 1968487
AN - SCOPUS:0025309796
VL - 144
SP - 1711
EP - 1720
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 5
ER -