Using robotic systems to process and embed colonic murine samples for histological analyses

Fulvia Milena Cribiú, Claudia Burrello, Roberta Tacchi, Francesca Boggio, Dario Ricca, Flavio Caprioli, Stefano Ferrero, Federica Facciotti

Research output: Contribution to journalArticle

Abstract

The understanding of human diseases has been greatly expanded thanks to the study of animal models. Nonetheless, histopathological evaluation of experimental models needs to be as rigorous as that applied for human samples. Indeed, drawing reliable and accurate conclusions is critically influenced by the quality of tissue section preparation. Here, we describe a protocol for histopathological analysis of murine tissues that implements several automated steps during the procedure, from the initial preparation to the paraffin embedding of the murine samples. The reduction of methodological variables through rigorous protocol standardization from automated procedures contributes to increased overall reliability of murine pathological analysis. Specifically, this protocol describes the utilization of automated processing and embedding robotic systems, routinely used for the tissue processing and paraffin embedding of human samples, to process murine specimens of intestinal inflammation. We conclude that the reliability of histopathological examination of murine tissues is significantly increased upon introduction of standardized and automated techniques.

Original languageEnglish
Article numbere58654
JournalJournal of Visualized Experiments
Volume2019
Issue number143
DOIs
Publication statusPublished - Jan 2019

Keywords

  • Automation
  • Embedding system
  • Histopathological analysis
  • Immunology and infection
  • Issue 143
  • Murine intestinal specimens
  • Murine models
  • Standardization

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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