Usnic acid, a natural antimicrobial agent able to inhibit bacterial biofilm formation on polymer surfaces

I. Francolini, P. Norris, A. Piozzi, G. Donelli, P. Stoodley

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

In modern medicine, artificial devices are used for repair or replacement of damaged parts of the body, delivery of drugs, and monitoring the status of critically ill patients. However, artificial surfaces are often susceptible to colonization by bacteria and fungi. Once microorganisms have adhered to the surface, they can form biofilms, resulting in highly resistant local or systemic infections. At this time, the evidence suggests that (+)-usnic acid, a secondary lichen metabolite, possesses antimicrobial activity against a number of planktonic gram-positive bacteria, including Staphylococcus aureus, Enterococcus faecalis, and Enterococcus faecium. Since lichens are surface-attached communities that produce antibiotics, including usnic acid, to protect themselves from colonization by other bacteria, we hypothesized that the mode of action of usnic acid may be utilized in the control of medical biofilms. We loaded (+)-usnic acid into modified polyurethane and quantitatively assessed the capacity of (+)-usnic acid to control biofilm formation by either S. aureus or Pseudomonas aeruginosa under laminar flow conditions by using image analysis. (+)-Usnic acid-loaded polymers did not inhibit the initial attachment of S. aureus cells, but killing the attacked cells resulted in the inhibition of biofilm. Interestingly, although P. aeruginosa biofilms did form on the surface of (+)-usnic acid-loaded polymer, the morphology of the biofilm was altered, possibly indicating that (+)-usnic acid interfered with signaling pathways.

Original languageEnglish
Pages (from-to)4360-4365
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume48
Issue number11
DOIs
Publication statusPublished - Nov 2004

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Biofilms
Anti-Infective Agents
Polymers
Staphylococcus aureus
Lichens
Pseudomonas aeruginosa
Bacteria
Enterococcus faecium
Modern 1601-history
Polyurethanes
usnic acid
Drug Monitoring
Enterococcus faecalis
Gram-Positive Bacteria
Human Body
Critical Illness
Fungi
Anti-Bacterial Agents
Equipment and Supplies
Infection

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Usnic acid, a natural antimicrobial agent able to inhibit bacterial biofilm formation on polymer surfaces. / Francolini, I.; Norris, P.; Piozzi, A.; Donelli, G.; Stoodley, P.

In: Antimicrobial Agents and Chemotherapy, Vol. 48, No. 11, 11.2004, p. 4360-4365.

Research output: Contribution to journalArticle

Francolini, I. ; Norris, P. ; Piozzi, A. ; Donelli, G. ; Stoodley, P. / Usnic acid, a natural antimicrobial agent able to inhibit bacterial biofilm formation on polymer surfaces. In: Antimicrobial Agents and Chemotherapy. 2004 ; Vol. 48, No. 11. pp. 4360-4365.
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