USP1 links platinum resistance to cancer cell dissemination by regulating Snail stability

Maura Sonego; Ilenia Pellarin; Alice Costa; Gian Luca Rampioni Vinciguerra; Michela Coan; Alexandra Kraut; Sara D'Andrea; Alessandra Dall'Acqua; Dan Cacsire Castillo-Tong; Daniela Califano; Simona Losito; Riccardo Spizzo; Yohann Couté; Andrea Vecchione; Barbara Belletti; Monica Schiappacassi; Gustavo Baldassarre

doi:10.1126/sciadv.aav3235

USP1 links platinum resistance to cancer cell dissemination by regulating Snail stability

Maura Sonego, Ilenia Pellarin, Alice Costa, Gian Luca Rampioni Vinciguerra, Michela Coan, Alexandra Kraut, Sara D'Andrea, Alessandra Dall'Acqua, Dan Cacsire Castillo-Tong, Daniela Califano, Simona Losito, Riccardo Spizzo, Yohann Couté, Andrea Vecchione, Barbara Belletti, Monica Schiappacassi, Gustavo Baldassarre

Istituto Nazionale Tumori Fondazione G. Pascale

Research output: Contribution to journal › Article

Abstract

Resistance to platinum-based chemotherapy is a common event in patients with cancer, generally associated with tumor dissemination and metastasis. Whether platinum treatment per se activates molecular pathways linked to tumor spreading is not known. Here, we report that the ubiquitin-specific protease 1 (USP1) mediates ovarian cancer cell resistance to platinum, by regulating the stability of Snail, which, in turn, promotes tumor dissemination. At the molecular level, we observed that upon platinum treatment, USP1 is phosphorylated by ATM and ATR and binds to Snail. Then, USP1 de-ubiquitinates and stabilizes Snail expression, conferring resistance to platinum, increased stem cell-like features, and metastatic ability. Consistently, knockout or pharmacological inhibition of USP1 increased platinum sensitivity and decreased metastatic dissemination in a Snail-dependent manner. Our findings identify Snail as a USP1 target and open the way to a novel strategy to overcome platinum resistance and more successfully treat patients with ovarian cancer.

Original language	English
Article number	eaav3235
Journal	Science Advances
Volume	5
Issue number	5
DOIs	https://doi.org/10.1126/sciadv.aav3235
Publication status	Published - Jan 1 2019

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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10.1126/sciadv.aav3235

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Sonego, M., Pellarin, I., Costa, A., Vinciguerra, G. L. R., Coan, M., Kraut, A., D'Andrea, S., Dall'Acqua, A., Castillo-Tong, D. C., Califano, D., Losito, S., Spizzo, R., Couté, Y., Vecchione, A., Belletti, B., Schiappacassi, M., & Baldassarre, G. (2019). USP1 links platinum resistance to cancer cell dissemination by regulating Snail stability. Science Advances, 5(5), [eaav3235]. https://doi.org/10.1126/sciadv.aav3235

USP1 links platinum resistance to cancer cell dissemination by regulating Snail stability. / Sonego, Maura; Pellarin, Ilenia; Costa, Alice; Vinciguerra, Gian Luca Rampioni; Coan, Michela; Kraut, Alexandra; D'Andrea, Sara; Dall'Acqua, Alessandra; Castillo-Tong, Dan Cacsire; Califano, Daniela ; Losito, Simona; Spizzo, Riccardo; Couté, Yohann; Vecchione, Andrea; Belletti, Barbara; Schiappacassi, Monica; Baldassarre, Gustavo.

In: Science Advances, Vol. 5, No. 5, eaav3235, 01.01.2019.

Research output: Contribution to journal › Article

Sonego, M, Pellarin, I, Costa, A, Vinciguerra, GLR, Coan, M, Kraut, A, D'Andrea, S, Dall'Acqua, A, Castillo-Tong, DC, Califano, D , Losito, S, Spizzo, R, Couté, Y, Vecchione, A, Belletti, B, Schiappacassi, M & Baldassarre, G 2019, 'USP1 links platinum resistance to cancer cell dissemination by regulating Snail stability', Science Advances, vol. 5, no. 5, eaav3235. https://doi.org/10.1126/sciadv.aav3235

Sonego, Maura ; Pellarin, Ilenia ; Costa, Alice ; Vinciguerra, Gian Luca Rampioni ; Coan, Michela ; Kraut, Alexandra ; D'Andrea, Sara ; Dall'Acqua, Alessandra ; Castillo-Tong, Dan Cacsire ; Califano, Daniela ; Losito, Simona ; Spizzo, Riccardo ; Couté, Yohann ; Vecchione, Andrea ; Belletti, Barbara ; Schiappacassi, Monica ; Baldassarre, Gustavo. / USP1 links platinum resistance to cancer cell dissemination by regulating Snail stability. In: Science Advances. 2019 ; Vol. 5, No. 5.

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abstract = "Resistance to platinum-based chemotherapy is a common event in patients with cancer, generally associated with tumor dissemination and metastasis. Whether platinum treatment per se activates molecular pathways linked to tumor spreading is not known. Here, we report that the ubiquitin-specific protease 1 (USP1) mediates ovarian cancer cell resistance to platinum, by regulating the stability of Snail, which, in turn, promotes tumor dissemination. At the molecular level, we observed that upon platinum treatment, USP1 is phosphorylated by ATM and ATR and binds to Snail. Then, USP1 de-ubiquitinates and stabilizes Snail expression, conferring resistance to platinum, increased stem cell-like features, and metastatic ability. Consistently, knockout or pharmacological inhibition of USP1 increased platinum sensitivity and decreased metastatic dissemination in a Snail-dependent manner. Our findings identify Snail as a USP1 target and open the way to a novel strategy to overcome platinum resistance and more successfully treat patients with ovarian cancer.",

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AU - Losito, Simona

AU - Spizzo, Riccardo

AU - Couté, Yohann

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