V3 loop core region serotyping of HIV-1 infected patients using the FHV epitope presenting system

M. Schiappacassi, E. Buratti, P. D'Agaro, L. Ciani, E. S. Scodeller, S. G. Tisminetzky, F. E. Baralle

Research output: Contribution to journalArticle

Abstract

We have reported recently a new epitope presenting system based on the Flock House Virus (FHV) capsid protein. The HIV-1 V3 loop core sequence IGPGRAF was inserted in different sites of this carrier molecule. Immunoreactivity experiments and molecular modelling consistently showed that the most reactive recombinant protein displayed the IGPGRAF sequence in a conformation which is most similar to that of a V3 loop reference structure. The same insertion site was then used to display the V3 loop apex sequences of six different HIV-1 isolates. Sera from 32 HIV-1 infected patients were examined for their reactivity to our chimeric proteins and the results were compared with those obtained using synthetic V3 loop peptides. The data obtained were confirmed by nested PCR amplification and direct sequencing of the patient's V3 loops. The results showed that the V3 loop serotyping using the FHV hybrid proteins, was more specific than that obtained using synthetic peptides. This system will therefore be a useful tool for the correct evaluation of the immune response against different V3 loop core sequences.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalJournal of Virological Methods
Volume63
Issue number1-2
DOIs
Publication statusPublished - Jan 1997

Keywords

  • FHV
  • HIV-1
  • V3 loop serotyping

ASJC Scopus subject areas

  • Virology

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    Schiappacassi, M., Buratti, E., D'Agaro, P., Ciani, L., Scodeller, E. S., Tisminetzky, S. G., & Baralle, F. E. (1997). V3 loop core region serotyping of HIV-1 infected patients using the FHV epitope presenting system. Journal of Virological Methods, 63(1-2), 121-127. https://doi.org/10.1016/S0166-0934(96)02120-9