Vacuolation induced by VacA toxin of Helicobacter pylori requires the intracellular accumulation of membrane permeant bases, Cl- and water

Laura Morbiato; Francesco Tombola; Silvia Campello; Giuseppe Del Giudice; Rino Rappuoli; Mario Zoratti; Emanuele Papini

doi:10.1016/S0014-5793(01)03133-7

Vacuolation induced by VacA toxin of Helicobacter pylori requires the intracellular accumulation of membrane permeant bases, Cl^- and water

Laura Morbiato, Francesco Tombola, Silvia Campello, Giuseppe Del Giudice, Rino Rappuoli, Mario Zoratti, Emanuele Papini

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

The protein vacuolating toxin A (VacA) of Helicobacter pylori converts late endosomes into large vacuoles in the presence of permeant bases. Here it is shown that this phenomenon corresponds to an accumulation of permeant bases and Cl^- in HeLa cells and requires the presence of extracellular Cl^-. The net influx of Cl^- is due to electroneutral, Na⁺, K⁺, 2Cl^- cotransporter-mediated transport. Cell vacuolation leads to cell volume increase, consistent with water flux into the cell, while hyper-osmotic media decreased vacuole formation. These data represent the first evidence that VacA-treated cells undergo an osmotic unbalance, reinforcing the hypothesis that the VacA chloride channel is responsible for cell vacuolation.

Original language	English
Pages (from-to)	479-483
Number of pages	5
Journal	FEBS Letters
Volume	508
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(01)03133-7
Publication status	Published - Nov 23 2001

Keywords

Cell vacuolation
Cl transport
Helicobacter pylori
Na, K, 2Cl cotransporter
VacA anion channel

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Access to Document

10.1016/S0014-5793(01)03133-7

Cite this

Vacuolation induced by VacA toxin of Helicobacter pylori requires the intracellular accumulation of membrane permeant bases, Cl^- and water. / Morbiato, Laura; Tombola, Francesco; Campello, Silvia; Del Giudice, Giuseppe; Rappuoli, Rino; Zoratti, Mario; Papini, Emanuele.

In: FEBS Letters, Vol. 508, No. 3, 23.11.2001, p. 479-483.

Research output: Contribution to journal › Article › peer-review

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abstract = "The protein vacuolating toxin A (VacA) of Helicobacter pylori converts late endosomes into large vacuoles in the presence of permeant bases. Here it is shown that this phenomenon corresponds to an accumulation of permeant bases and Cl- in HeLa cells and requires the presence of extracellular Cl-. The net influx of Cl- is due to electroneutral, Na+, K+, 2Cl- cotransporter-mediated transport. Cell vacuolation leads to cell volume increase, consistent with water flux into the cell, while hyper-osmotic media decreased vacuole formation. These data represent the first evidence that VacA-treated cells undergo an osmotic unbalance, reinforcing the hypothesis that the VacA chloride channel is responsible for cell vacuolation.",

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AU - Del Giudice, Giuseppe

AU - Rappuoli, Rino

AU - Zoratti, Mario

AU - Papini, Emanuele

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