TY - JOUR
T1 - Vacuolation induced by VacA toxin of Helicobacter pylori requires the intracellular accumulation of membrane permeant bases, Cl- and water
AU - Morbiato, Laura
AU - Tombola, Francesco
AU - Campello, Silvia
AU - Del Giudice, Giuseppe
AU - Rappuoli, Rino
AU - Zoratti, Mario
AU - Papini, Emanuele
PY - 2001/11/23
Y1 - 2001/11/23
N2 - The protein vacuolating toxin A (VacA) of Helicobacter pylori converts late endosomes into large vacuoles in the presence of permeant bases. Here it is shown that this phenomenon corresponds to an accumulation of permeant bases and Cl- in HeLa cells and requires the presence of extracellular Cl-. The net influx of Cl- is due to electroneutral, Na+, K+, 2Cl- cotransporter-mediated transport. Cell vacuolation leads to cell volume increase, consistent with water flux into the cell, while hyper-osmotic media decreased vacuole formation. These data represent the first evidence that VacA-treated cells undergo an osmotic unbalance, reinforcing the hypothesis that the VacA chloride channel is responsible for cell vacuolation.
AB - The protein vacuolating toxin A (VacA) of Helicobacter pylori converts late endosomes into large vacuoles in the presence of permeant bases. Here it is shown that this phenomenon corresponds to an accumulation of permeant bases and Cl- in HeLa cells and requires the presence of extracellular Cl-. The net influx of Cl- is due to electroneutral, Na+, K+, 2Cl- cotransporter-mediated transport. Cell vacuolation leads to cell volume increase, consistent with water flux into the cell, while hyper-osmotic media decreased vacuole formation. These data represent the first evidence that VacA-treated cells undergo an osmotic unbalance, reinforcing the hypothesis that the VacA chloride channel is responsible for cell vacuolation.
KW - Cell vacuolation
KW - Cl transport
KW - Helicobacter pylori
KW - Na, K, 2Cl cotransporter
KW - VacA anion channel
UR - http://www.scopus.com/inward/record.url?scp=0035941065&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035941065&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(01)03133-7
DO - 10.1016/S0014-5793(01)03133-7
M3 - Article
C2 - 11728476
AN - SCOPUS:0035941065
VL - 508
SP - 479
EP - 483
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -