Validation of 8-isoprostane and prostaglandin E2 measurements in exhaled breath condensate

P. Montuschi, E. Ragazzoni, S. Valente, G. Corbo, C. Mondino, G. Ciappi, G. Ciabattoni

Research output: Contribution to journalArticle

Abstract

Objectives: To qualitatively validate radioimmunoassays for 8-isoprostane and prostaglandin (PG) E2 in exhaled breath condensate. Subjects: Twenty-two subjects with different lung diseases attended the outpatient clinic on one occasion for exhaled breath condensate collection. Methods. Samples were pooled together and purified by reverse phase high performance liquid chromatography (RP-HPLC). The eluted fractions were assayed for 8-isoprostane-like immunoreactivity and PGE2-like immunoreactivity by radioimmunoassays. In addition, simultaneous measurements of exhaled breath condensate unextracted samples with two anti-8-isoprostane and anti-PGE 2 sera with different cross-reactivity were performed. Results: A single peak of 8-isoprostane-like immunoreactivity and PGE2-like immunoreactivity co-eluting with 8-isoprostane (retention time: 13 min) and PGE2 (retention time: 21 min) standards, respectively, was identified by radioimmunoassays. Testing with two different antisera showed similar results for both 8-isoprostane-like immunoreactivity (limits of agreement = 4.5 pg/ml and -4.1 pg/ml, n = 12) and PGE2-like immunoreactivity (limits of agreement = 6.1 pg/ ml and - 6.1 pg/ml, n = 12). Conclusions: This study provides evidence for the specificity of the radioimmunoassays for 8-isoprostane and PGE2 in exhaled breath condensate. This is critical for proposing these markers as a non-invasive way for monitoring airway inflammation.

Original languageEnglish
Pages (from-to)502-507
Number of pages6
JournalInflammation Research
Volume52
Issue number12
DOIs
Publication statusPublished - Dec 2003

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Keywords

  • 8-Isoprostane, prostaglandin E
  • Airway inflammation
  • Exhaled breath condensate
  • Radioimmunoassay

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)
  • Immunology
  • Cell Biology

Cite this

Montuschi, P., Ragazzoni, E., Valente, S., Corbo, G., Mondino, C., Ciappi, G., & Ciabattoni, G. (2003). Validation of 8-isoprostane and prostaglandin E2 measurements in exhaled breath condensate. Inflammation Research, 52(12), 502-507. https://doi.org/10.1007/s00011-003-1212-6