Validation of Alzheimer's disease CSF and plasma biological markers: The multicentre reliability study of the pilot European Alzheimer's Disease Neuroimaging Initiative (E-ADNI)

Katharina Buerger, Giovanni Frisoni, Olga Uspenskaya, Michael Ewers, Henrik Zetterberg, Cristina Geroldi, Giuliano Binetti, Peter Johannsen, Paolo Maria Rossini, Lars Olof Wahlund, Bruno Vellas, Kaj Blennow, Harald Hampel

Research output: Contribution to journalArticle

Abstract

Background: Alzheimer's Disease Neuroimaging Initiatives ("ADNI") aim to validate neuroimaging and biochemical markers of Alzheimer's disease (AD). Data of the pilot European-ADNI (E-ADNI) biological marker programme of cerebrospinal fluid (CSF) and plasma candidate biomarkers are reported. Methods: Six academic EADC centres recruited 49 subjects (healthy controls, subjects with mild cognitive impairment (MCI) and AD). We measured CSF β-amyloid 42 (CSF Aβ42), total tau-protein (t-tau), phosphorylated tau-proteins (P-tau181, P-tau231), plasma β-amyloid 40 and 42 (Aβ40/Aβ42). Immediate fresh shipment was compared to freezing and later shipment on dry ice. Results: CSF T-tau (fresh samples) was increased in AD versus controls (p = 0.049), CSF Aβ42 (frozen samples) was decreased in MCI and AD (p = 0.02), as well as plasma Aβ40 (fresh and frozen samples) in AD (p = 0.049 and p = 0.016). Pooled values of neurochemical parameters and ratios thereof were different between centres (p <0.005). Analysis of frozen samples yielded higher diagnostic accuracy than immediate fresh shipment with 100% (fresh: 100%) correctly classified in control subjects, 100% (78%) in MCI, 91% (91%) in AD. Conclusion: The use of frozen rather than fresh samples renders higher diagnostic accuracy within a multicentre context. We confirmed the feasibility of a multicentre AD biomarker programme for future clinical trials.

Original languageEnglish
Pages (from-to)579-585
Number of pages7
JournalExperimental Gerontology
Volume44
Issue number9
DOIs
Publication statusPublished - Sep 2009

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Keywords

  • Alzheimer's disease (AD)
  • Alzheimer's Disease Neuroimaging Initiative (ADNI)
  • Biological marker
  • Mild cognitive impairment (MCI)
  • Validation

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Cell Biology
  • Endocrinology
  • Genetics
  • Molecular Biology

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