Validation of leukotriene B4 measurements in exhaled breath condensate

P. Montuschi, E. Ragazzoni, S. Valente, G. Corbo, C. Mondino, G. Ciappi, P. J. Barnes, G. Ciabattoni

Research output: Contribution to journalArticle

Abstract

Objective: To qualitatively validate an enzyme immunoassay to measure leukotriene B4 in exhaled breath condensate. Exhaled breath condensate is a new non-invasive method to monitor airway inflammation. Subjects: Twenty-two subjects with different lung diseases attended the outpatient clinic on one occasion for exhaled breath condensate collection. Methods: Samples were pooled together and purified by reverse-phase high-performance liquid chromatography. The fractions eluted were assayed for leukotriene B4 by enzyme immunoassay. Results: A single peak of leukotriene B4-like immunoreactivity co-eluting with leukotriene B4 standard (retention time: 24 min) was identified by enzyme immunoassay. Reverse phase-high performance liquid chromatography peak of leukotriene B4 was clearly separated from those of 6-transleukotriene B4 (retention time: 14 min) and leukotriene B5 (retention time: 18 min) for which the antiserum used in the enzyme immunoassay had the highest cross-reactivity. Leukotriene B4 recovery was 64%. Conclusions: This study provides evidence for the presence of leukotriene B4 in the exhaled breath condensate and the specificity of the enzyme immunoassay used.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalInflammation Research
Volume52
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

Keywords

  • Airway inflammation
  • Enzyme immunoassay
  • Exhaled breath condensate
  • Leukotriene B
  • Reverse-phase high-performance liquid chromatography

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)
  • Immunology
  • Cell Biology

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  • Cite this

    Montuschi, P., Ragazzoni, E., Valente, S., Corbo, G., Mondino, C., Ciappi, G., Barnes, P. J., & Ciabattoni, G. (2003). Validation of leukotriene B4 measurements in exhaled breath condensate. Inflammation Research, 52(2), 69-73. https://doi.org/10.1007/s000110300003