Validation of leukotriene B4 measurements in exhaled breath condensate

P. Montuschi, E. Ragazzoni, S. Valente, G. Corbo, C. Mondino, G. Ciappi, P. J. Barnes, G. Ciabattoni

Research output: Contribution to journalArticle

Abstract

Objective: To qualitatively validate an enzyme immunoassay to measure leukotriene B4 in exhaled breath condensate. Exhaled breath condensate is a new non-invasive method to monitor airway inflammation. Subjects: Twenty-two subjects with different lung diseases attended the outpatient clinic on one occasion for exhaled breath condensate collection. Methods: Samples were pooled together and purified by reverse-phase high-performance liquid chromatography. The fractions eluted were assayed for leukotriene B4 by enzyme immunoassay. Results: A single peak of leukotriene B4-like immunoreactivity co-eluting with leukotriene B4 standard (retention time: 24 min) was identified by enzyme immunoassay. Reverse phase-high performance liquid chromatography peak of leukotriene B4 was clearly separated from those of 6-transleukotriene B4 (retention time: 14 min) and leukotriene B5 (retention time: 18 min) for which the antiserum used in the enzyme immunoassay had the highest cross-reactivity. Leukotriene B4 recovery was 64%. Conclusions: This study provides evidence for the presence of leukotriene B4 in the exhaled breath condensate and the specificity of the enzyme immunoassay used.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalInflammation Research
Volume52
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

Fingerprint

Leukotriene B4
Immunoenzyme Techniques
Enzymes
High performance liquid chromatography
High Pressure Liquid Chromatography
Pulmonary diseases
Reverse-Phase Chromatography
Ambulatory Care Facilities
Lung Diseases
Immune Sera
Inflammation
Recovery

Keywords

  • Airway inflammation
  • Enzyme immunoassay
  • Exhaled breath condensate
  • Leukotriene B
  • Reverse-phase high-performance liquid chromatography

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)
  • Immunology
  • Cell Biology

Cite this

Montuschi, P., Ragazzoni, E., Valente, S., Corbo, G., Mondino, C., Ciappi, G., ... Ciabattoni, G. (2003). Validation of leukotriene B4 measurements in exhaled breath condensate. Inflammation Research, 52(2), 69-73. https://doi.org/10.1007/s000110300003

Validation of leukotriene B4 measurements in exhaled breath condensate. / Montuschi, P.; Ragazzoni, E.; Valente, S.; Corbo, G.; Mondino, C.; Ciappi, G.; Barnes, P. J.; Ciabattoni, G.

In: Inflammation Research, Vol. 52, No. 2, 01.02.2003, p. 69-73.

Research output: Contribution to journalArticle

Montuschi, P, Ragazzoni, E, Valente, S, Corbo, G, Mondino, C, Ciappi, G, Barnes, PJ & Ciabattoni, G 2003, 'Validation of leukotriene B4 measurements in exhaled breath condensate', Inflammation Research, vol. 52, no. 2, pp. 69-73. https://doi.org/10.1007/s000110300003
Montuschi P, Ragazzoni E, Valente S, Corbo G, Mondino C, Ciappi G et al. Validation of leukotriene B4 measurements in exhaled breath condensate. Inflammation Research. 2003 Feb 1;52(2):69-73. https://doi.org/10.1007/s000110300003
Montuschi, P. ; Ragazzoni, E. ; Valente, S. ; Corbo, G. ; Mondino, C. ; Ciappi, G. ; Barnes, P. J. ; Ciabattoni, G. / Validation of leukotriene B4 measurements in exhaled breath condensate. In: Inflammation Research. 2003 ; Vol. 52, No. 2. pp. 69-73.
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