Validation of micronuclei frequency in peripheral blood lymphocytes as early cancer risk biomarker in a nested case-control study

Elena Murgia, Michela Ballardin, Stefano Bonassi, Anna Maria Rossi, Roberto Barale

Research output: Contribution to journalArticlepeer-review

Abstract

Aim of this work was to assess the predictive value of micronuclei (MN) frequency in peripheral blood lymphocytes (PBL) for the risk of cancer death in disease-free individuals. Blood samples from 1650 subjects selected from the general population of Pisa, Italy, were collected between June 1991 and November 1993. The follow-up until January 2005 recorded a total of 111 deaths (52 for cancer). MN frequency was assessed for 49 cancer cases and 101 matched controls. A significantly higher MN frequency was found in cancer cases (4.7 ± 3.4 MN/1000 BN cells) versus controls (1.5 ± 1.7; p <0.0001). Donors were stratified in two classes and multivariate logistic regression analysis confirmed that individuals with high MN frequency (>2.5 MN/1000 BN cells) had a significantly increased risk of cancer death (OR = 10.7; 95% CI = 4.6-24.9; p <0.0001) when compared to individuals with low MN frequency (≤2.5 MN/1000 BN cells). Ageing was associated with a 6% increased risk per year (p = 0.03). No influence of other potential confounders (gender, occupation, smoking and drinking habits) was observed. Finally, subjects with a higher MN frequency showed a higher MR for CVD (Logrank test, p = 0.001). These findings provide strong evidence that MN frequency assessed in PBL of disease-free subjects is a good predictor of cancer death risk, evaluated by a nested case-control study performed 14 years after the original recruitment.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume639
Issue number1-2
DOIs
Publication statusPublished - Mar 1 2008

Keywords

  • Biological markers
  • Cancer risk
  • Case-control study
  • Micronucleus test
  • Validation

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Molecular Biology

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