Validity and reproducibility of morphologic analysis of nasal secretions obtained using ultrasonic nebulization of hypertonic solution

Maria T. Ventura, Olga Toungoussova, Maria P. Foschino Barbaro, Onofrio Resta, Giovanna E. Carpagnano, Silvano Dragonieri, Giovanni B. Migliori, Margherita Neri, Antonio Spanevello

Research output: Contribution to journalArticle

Abstract

Background: Collection of nasal secretions is important for the evaluation of upper airways inflammation in many nasal disorders. Objective: To study the validity and reproducibility of nasal secretion cellularity induced by nebulization of hypertonic solution in patients with allergic rhinitis (AR), patients with nonallergic rhinitis with eosinophilia syndrome (NARES), and control subjects. Methods: Sixty-eight individuals (29 with AR [mean ± SD age, 33.3 ± 16.9 years], 23 with NARES [mean ± SD age, 46.4 ± 16.6 years], and 16 controls [mean ± SD age, 42.1 ±1 5.1 years]) underwent ultrasonic nebulization of hypertonic (4.5%) saline solution on 2 different occasions to study the validity and reproducibility of total and differential cell counts of nasal secretions. Results: The mean ± SD percentage of eosinophils was significantly higher in samples from patients with AR (20.8% ± 23.1%) and NARES (18.7% ± 22.8%) than in samples from controls (0.6% ± 0.6%; P <.001 for both). There was a significant correlation between 2 samples of nasal secretions obtained on 2 different occasions for percentages of macrophages, neutrophils, eosinophils, and epithelial cells. Conclusions: The analysis of nasal secretions obtained using ultrasonic nebulization of hypertonic solution can distinguish patients with AR and NARES from controls. The reproducibility of this technique is good for macrophages, neutrophils, eosinophils, and epithelial cells. This method could be used to detect nasal airway inflammation in clinical settings.

Original languageEnglish
Pages (from-to)232-235
Number of pages4
JournalAnnals of Allergy, Asthma and Immunology
Volume99
Issue number3
Publication statusPublished - Sep 2007

ASJC Scopus subject areas

  • Immunology and Allergy

Fingerprint Dive into the research topics of 'Validity and reproducibility of morphologic analysis of nasal secretions obtained using ultrasonic nebulization of hypertonic solution'. Together they form a unique fingerprint.

  • Cite this

    Ventura, M. T., Toungoussova, O., Foschino Barbaro, M. P., Resta, O., Carpagnano, G. E., Dragonieri, S., Migliori, G. B., Neri, M., & Spanevello, A. (2007). Validity and reproducibility of morphologic analysis of nasal secretions obtained using ultrasonic nebulization of hypertonic solution. Annals of Allergy, Asthma and Immunology, 99(3), 232-235.