Validity for epidemiological studies of long-term cryoconservation of steroid and protein hormones in serum and plasma

G. Bolelli, P. Muti, A. Micheli, R. Sciajno, F. Franceschetti, V. Krogh, P. Pisani, F. Berrino

Research output: Contribution to journalArticle

Abstract

Prospective studies based on the storage of biological samples at low temperature have opened new perspectives in etiological research on cancer. In planning these studies a crucial question is to evaluate whether the long- term preservation of the samples is able to affect the categorization of the subjects involved. In the frame of the ORDET project, a prospective study of hormones and diet in the etiology of breast cancer provided with a -80°C biological bank, we have evaluated the stability of estradiol, free and total testosterone, progesterone, and prolactin in serum and plasma samples over 3 years of cryoconservation. Study results showed that the subjects maintained almost the same rank by hormonal concentration throughout the 3-year period for all hormones. Looking at the stability over time, estradiol, prolactin, and total testosterone had fairly good performance for both serum and plasma. Serum-free testosterone increased in time up to 30%, whereas progesterone decreased by about 4.0% of the initial concentration. However, the reliability of the individual categorization by hormonal level suggests the validity of low temperature storage for epidemiological purposes, at least for hormonal parameters.

Original languageEnglish
Pages (from-to)509-513
Number of pages5
JournalCancer Epidemiology Biomarkers and Prevention
Volume4
Issue number5
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

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    Bolelli, G., Muti, P., Micheli, A., Sciajno, R., Franceschetti, F., Krogh, V., Pisani, P., & Berrino, F. (1995). Validity for epidemiological studies of long-term cryoconservation of steroid and protein hormones in serum and plasma. Cancer Epidemiology Biomarkers and Prevention, 4(5), 509-513.