Value of a commercial kit for detecting anti-C1q autoantibodies and correlation with immunological and clinical activity of lupus nephritis

Federica De Liso, Caterina Matinato, Cristina Novembrino, Antonella Radice, Francesca Raffiotta, Mariangela Ronchi, Maria Daniela Croci, Renato Alberto Sinico, Piergiorgio Messa, Erminio Torresani, Gabriella Moroni

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: The association of anti-C1q antibodies (anti-C1q) with the renal activity of lupus nephritis (LN) and the methods for their determination is still a matter of debate. Methods: In 116 serum samples of 66 patients with biopsy proven LN, we aimed: 1) to compare the results of the determination of anti-C1q obtained by a commercial kit with a clinically validated in-house ELISA; 2) to evaluate the correlation of anti-C1q with the most important immunological and clinical parameters employed in LN, i.e., antibodies to dsDNA (anti-dsDNA), C3 and C4 complement component, haemoglobin and haematuria. Results: Good correlation and agreement between the two methods (r=0.81, p

Original languageEnglish
Pages (from-to)1771-1777
Number of pages7
JournalClinical Chemistry and Laboratory Medicine
Volume53
Issue number11
DOIs
Publication statusPublished - Oct 1 2015

Fingerprint

Complement C4
Lupus Nephritis
Biopsy
Autoantibodies
Anti-Idiotypic Antibodies
Hemoglobins
Antibodies
Hematuria
Enzyme-Linked Immunosorbent Assay
Kidney
Serum

Keywords

  • anti-C1q autoantibodies
  • lupus nephritis
  • method comparison

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Value of a commercial kit for detecting anti-C1q autoantibodies and correlation with immunological and clinical activity of lupus nephritis. / De Liso, Federica; Matinato, Caterina; Novembrino, Cristina; Radice, Antonella; Raffiotta, Francesca; Ronchi, Mariangela; Croci, Maria Daniela; Sinico, Renato Alberto; Messa, Piergiorgio; Torresani, Erminio; Moroni, Gabriella.

In: Clinical Chemistry and Laboratory Medicine, Vol. 53, No. 11, 01.10.2015, p. 1771-1777.

Research output: Contribution to journalArticle

De Liso, Federica ; Matinato, Caterina ; Novembrino, Cristina ; Radice, Antonella ; Raffiotta, Francesca ; Ronchi, Mariangela ; Croci, Maria Daniela ; Sinico, Renato Alberto ; Messa, Piergiorgio ; Torresani, Erminio ; Moroni, Gabriella. / Value of a commercial kit for detecting anti-C1q autoantibodies and correlation with immunological and clinical activity of lupus nephritis. In: Clinical Chemistry and Laboratory Medicine. 2015 ; Vol. 53, No. 11. pp. 1771-1777.
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AU - Ronchi, Mariangela

AU - Croci, Maria Daniela

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