Value of immunohistochemical detection of noncollagenous proteins of bone for the diagnosis of bone tumours

M. Serra, K. Scotlandi, M. R. Sollazzo, M. Sarti, D. Maurici, S. Benini, P. Picci, Franco Bertoni, N. Baldini

Research output: Contribution to journalArticlepeer-review

Abstract

The expression of osteonectin, osteopontin, bone sialoprotein, and osteocalcin was evaluated by immunohistochemistry in 57 cases of osteoid-forming and nonosteoid-forming bone tumours using specific polyclonal antibodies and the avidin-biotin peroxidase complex method. A positive immunostaining was found in all of the osteoid-forming tumours (osteoblastoma and osteosarcoma), both in the cells and in the extracelullar matrix. Among non-osteoid-forming tumours, immunoreactivity to noncollagenous proteins was present in the cells but not in the matrix of chondrosarcoma, malignant fibrous histiocytoma, and fibrosarcoma, as well as in the mononuclear component of giant-cell tumours. Contrary to small-cell osteosarcoma, Ewing's sarcoma was always negative for all of the noncollagenous proteins considered. These results suggest that the immunohistochemical evaluation of noncollagenous proteins of bone may be a useful tool for the differential diagnosis of bone neoplasms, particularly among the heterogeneous group of small round cell tumours.

Original languageEnglish
Pages (from-to)257-261
Number of pages5
JournalInternational Journal of Oncology
Volume9
Issue number2
Publication statusPublished - Aug 1996

Keywords

  • Bone sialoprotein
  • Bone tumours
  • Immunohistochemistry
  • Osteocalcin
  • Osteonectin
  • Osteopontin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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