VAMP subfamilies identified by specific R-SNARE motifs

Valeria Rossi, Raffaella Picco, Marcella Vacca, Maurizio D'Esposito, Michele D'Urso, Thierry Galli, Francesco Filippini

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In eukaryotes, interactions among the α-helical coiled-coil domains (CCDs) of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) play a pivotal role in mediating the fusion among vesicles and target membranes. Surface residues of such CCDs are major candidates to regulate the specificity of membrane fusion, as they may alter local charge at the interaction layers and surface of the fusion complex, possibly modulating its formation and/or the binding of non-SNARE regulatory factors. Based on alternate patterns in surface residues, we have identified two motifs which group vesicular SNAREs in two novel subfamilies: RG-SNAREs and RD-SNAREs. The RG-SNARE CCD is common to all members of the widely conserved family of long VAMPs or longins and to yeast and non-neuronal VAMPs, possibly mediating "basic" fusion mechanisms; instead, only synaptobrevins from Bilateria share an RD-SNARE CCD, which is likely to mediate interactions to specific, yet unknown, regulatory factors and/or be the landmark of rapid fusion reactions like that mediating the release of neurotransmitters.

Original languageEnglish
Pages (from-to)251-256
Number of pages6
JournalBiology of the cell / under the auspices of the European Cell Biology Organization
Volume96
Issue number4
DOIs
Publication statusPublished - May 2004

Fingerprint

R388
Amino Acid Motifs
Proteins
R-SNARE Proteins
SNARE Proteins
Membrane Fusion
Eukaryota
Neurotransmitter Agents
Yeasts
Membranes

Keywords

  • Coiled-coil domain
  • Longin
  • PROSITE signature
  • Synaptobrevin

ASJC Scopus subject areas

  • Cell Biology

Cite this

VAMP subfamilies identified by specific R-SNARE motifs. / Rossi, Valeria; Picco, Raffaella; Vacca, Marcella; D'Esposito, Maurizio; D'Urso, Michele; Galli, Thierry; Filippini, Francesco.

In: Biology of the cell / under the auspices of the European Cell Biology Organization, Vol. 96, No. 4, 05.2004, p. 251-256.

Research output: Contribution to journalArticle

Rossi, Valeria ; Picco, Raffaella ; Vacca, Marcella ; D'Esposito, Maurizio ; D'Urso, Michele ; Galli, Thierry ; Filippini, Francesco. / VAMP subfamilies identified by specific R-SNARE motifs. In: Biology of the cell / under the auspices of the European Cell Biology Organization. 2004 ; Vol. 96, No. 4. pp. 251-256.
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