Vanilloid TRPV1 receptor mediates the antihyperalgesic effect of the nonpsychoactive cannabinoid, cannabidiol, in a rat model of acute inflammation

Barbara Costa, Gabriella Giagnoni, Chiara Franke, Anna Elisa Trovato, Mariapia Colleoni

Research output: Contribution to journalArticlepeer-review

Abstract

Cannabidiol (CBD), a nonpsychoactive marijuana constituent, was recently shown as an oral antihyperalgesic compound in a rat model of acute inflammation. We examined whether the CBD antihyperalgesic effect could be mediated by cannabinoid receptor type 1 (CB1) or cannabinoid receptor type 2 (CB2) and/or by transient receptor potential vanilloid type 1 (TRPV1). Rats received CBD (10 mg kg -1) and the selective antagonists: SR141716 (N-(piperidin-1-yl)-5- (4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide) for CB1, SR144528 (N-[(1S)-endo-1,3,3-trimethylbicyclo[2.2.1]heptan-2-yl]-5-(4- chloro-3-methylphenyl)-1-(4-methylbenzyl)pyrazole-3 carboxamide) for CB2 and capsazepine (CPZ) for TRPV1 receptors. The intraplantar injection of carrageenan in rats induced a time-dependent thermal hyperalgesia, which peaked at 3 h and decreased at the following times. CBD, administered 2 h after carrageenan, abolished the hyperalgesia to the thermal stimulus evaluated by plantar test. Neither SR141716 (0.5 mg kg -1) nor SR144528 (3 and 10 mg kg -1) modified the CBD-induced antihyperalgesia; CPZ partially at the lowest dose (2 mg kg -1) and fully at the highest dose (10 mg kg -1) reversed this effect. These results demonstrate that TRPV1 receptor could be a molecular target of the CBD antihyperalgesic action.

Original languageEnglish
Pages (from-to)247-250
Number of pages4
JournalBritish Journal of Pharmacology
Volume143
Issue number2
DOIs
Publication statusPublished - Sep 2004

Keywords

  • Cannabidiol
  • Cannabinoid
  • Carrageenan
  • Hyperalgesia
  • TRPV1

ASJC Scopus subject areas

  • Pharmacology

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