Variability in immunohistochemical detection of programmed death ligand 1 (PD-l1) in cancer tissue types

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21 Citations (Scopus)

Abstract

In normal cell physiology, programmed death 1 (PD-1) and its ligand, PD-L1, play an immunoregulatory role in T-cell activation, tolerance, and immune-mediated tissue damage. The PD-1/PD-L1 pathway also plays a critical role in immune escape of tumor cells and has been demonstrated to correlate with a poor prognosis of patients with several types of cancer. However, recent reports have revealed that the immunohistochemical (IHC) expression of the PD-L1 in tumor cells is not uniform for the use of different antibodies clones, with variable specificity, often doubtful topographical localization, and with a score not uniquely defined. The purpose of this study was to analyze the IHC expression of PD-L1 on a large series of several human tumors to correctly define its staining in different tumor tissues.

Original languageEnglish
JournalInternational Journal of Molecular Sciences
Volume17
Issue number5
DOIs
Publication statusPublished - May 21 2016

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death
Tumors
tumors
cancer
Ligands
Tissue
ligands
Neoplasms
Cells
cytology
Tumor Escape
Cell Physiological Phenomena
Immune Tolerance
T-cells
prognosis
Physiology
staining
antibodies
Antibodies
escape

Keywords

  • Immunohistochemical staining
  • PD-L1
  • Tissue type’s variability

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

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title = "Variability in immunohistochemical detection of programmed death ligand 1 (PD-l1) in cancer tissue types",
abstract = "In normal cell physiology, programmed death 1 (PD-1) and its ligand, PD-L1, play an immunoregulatory role in T-cell activation, tolerance, and immune-mediated tissue damage. The PD-1/PD-L1 pathway also plays a critical role in immune escape of tumor cells and has been demonstrated to correlate with a poor prognosis of patients with several types of cancer. However, recent reports have revealed that the immunohistochemical (IHC) expression of the PD-L1 in tumor cells is not uniform for the use of different antibodies clones, with variable specificity, often doubtful topographical localization, and with a score not uniquely defined. The purpose of this study was to analyze the IHC expression of PD-L1 on a large series of several human tumors to correctly define its staining in different tumor tissues.",
keywords = "Immunohistochemical staining, PD-L1, Tissue type’s variability",
author = "Giosu{\`e} Scognamiglio and {De Chiara}, {Anna Rosaria} and {Di Bonito}, Maurizio and Fabiana Tatangelo and Losito, {Nunzia Simona} and Annamaria Anniciello and {De Cecio}, Rossella and Crescenzo D’Alterio and Stefania Scala and Monica Cantile and Gerardo Botti",
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month = "5",
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language = "English",
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T1 - Variability in immunohistochemical detection of programmed death ligand 1 (PD-l1) in cancer tissue types

AU - Scognamiglio, Giosuè

AU - De Chiara, Anna Rosaria

AU - Di Bonito, Maurizio

AU - Tatangelo, Fabiana

AU - Losito, Nunzia Simona

AU - Anniciello, Annamaria

AU - De Cecio, Rossella

AU - D’Alterio, Crescenzo

AU - Scala, Stefania

AU - Cantile, Monica

AU - Botti, Gerardo

PY - 2016/5/21

Y1 - 2016/5/21

N2 - In normal cell physiology, programmed death 1 (PD-1) and its ligand, PD-L1, play an immunoregulatory role in T-cell activation, tolerance, and immune-mediated tissue damage. The PD-1/PD-L1 pathway also plays a critical role in immune escape of tumor cells and has been demonstrated to correlate with a poor prognosis of patients with several types of cancer. However, recent reports have revealed that the immunohistochemical (IHC) expression of the PD-L1 in tumor cells is not uniform for the use of different antibodies clones, with variable specificity, often doubtful topographical localization, and with a score not uniquely defined. The purpose of this study was to analyze the IHC expression of PD-L1 on a large series of several human tumors to correctly define its staining in different tumor tissues.

AB - In normal cell physiology, programmed death 1 (PD-1) and its ligand, PD-L1, play an immunoregulatory role in T-cell activation, tolerance, and immune-mediated tissue damage. The PD-1/PD-L1 pathway also plays a critical role in immune escape of tumor cells and has been demonstrated to correlate with a poor prognosis of patients with several types of cancer. However, recent reports have revealed that the immunohistochemical (IHC) expression of the PD-L1 in tumor cells is not uniform for the use of different antibodies clones, with variable specificity, often doubtful topographical localization, and with a score not uniquely defined. The purpose of this study was to analyze the IHC expression of PD-L1 on a large series of several human tumors to correctly define its staining in different tumor tissues.

KW - Immunohistochemical staining

KW - PD-L1

KW - Tissue type’s variability

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DO - 10.3390/ijms17050790

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