Variability OF HIV-1 V2 env domain for integrin binding: Clinical correlates

Claudia Fabrizio; Davide F. Bavaro; Luigia Scudeller; Luciana Lepore; Flavia Balena; Antonella Lagioia; Gioacchino Angarano; L. Monno; Annalisa Saracino

doi:10.1016/j.virol.2019.07.019

Variability OF HIV-1 V2 env domain for integrin binding: Clinical correlates

Claudia Fabrizio, Davide F. Bavaro, Luigia Scudeller, Luciana Lepore, Flavia Balena, Antonella Lagioia, Gioacchino Angarano, L. Monno, Annalisa Saracino

Research output: Contribution to journal › Article

Abstract

The HIV V2^179−181 (HXB2 numbering) tripeptide mediates binding to α4β7 integrin, which is responsible for GALT homing. Our study aimed to assess V2 variability in naive HIV-1 infected patients and its association with clinical and viro-immunological features. Gp120 sequences were obtained from 322 subjects; length, potential N-linked glycosylation sites (PNGs), net-charge (NC) and ¹⁷⁹⁻ ¹⁸¹tripeptide α4β7-binding-motif of V2 were evaluated. At multivariate analysis, lower V2 length and higher NC correlated with low CD4 cells; no association was found with PNGs. A greater variability pertained positions 162–163, 164–167, 169, 175–179, 187, 194 and 195 in B sequences, and 163 and 177 in X4 tropic viruses. LDV was the most common tripeptide. Asp¹⁸⁰ was highly conserved; Leu¹⁷⁹ was more frequently observed in non-B and in recent infections compared to others, while Val¹⁸¹ was found in recent infections and in MSM. Further studies to deeply explore the clinical significance of these associations are warranted.

Original language	English
Pages (from-to)	266-271
Number of pages	6
Journal	Virology
Volume	535
DOIs	https://doi.org/10.1016/j.virol.2019.07.019
Publication status	Published - Sep 1 2019

Keywords

Gp-120
HIV-1
Integrins
V2 tripeptide
α4β7

ASJC Scopus subject areas

Virology

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Fabrizio, C., Bavaro, D. F., Scudeller, L., Lepore, L., Balena, F., Lagioia, A., Angarano, G., Monno, L., & Saracino, A. (2019). Variability OF HIV-1 V2 env domain for integrin binding: Clinical correlates. Virology, 535, 266-271. https://doi.org/10.1016/j.virol.2019.07.019

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title = "Variability OF HIV-1 V2 env domain for integrin binding: Clinical correlates",

abstract = "The HIV V2179−181 (HXB2 numbering) tripeptide mediates binding to α4β7 integrin, which is responsible for GALT homing. Our study aimed to assess V2 variability in naive HIV-1 infected patients and its association with clinical and viro-immunological features. Gp120 sequences were obtained from 322 subjects; length, potential N-linked glycosylation sites (PNGs), net-charge (NC) and 179− 181tripeptide α4β7-binding-motif of V2 were evaluated. At multivariate analysis, lower V2 length and higher NC correlated with low CD4 cells; no association was found with PNGs. A greater variability pertained positions 162–163, 164–167, 169, 175–179, 187, 194 and 195 in B sequences, and 163 and 177 in X4 tropic viruses. LDV was the most common tripeptide. Asp180 was highly conserved; Leu179 was more frequently observed in non-B and in recent infections compared to others, while Val181 was found in recent infections and in MSM. Further studies to deeply explore the clinical significance of these associations are warranted.",

keywords = "Gp-120, HIV-1, Integrins, V2 tripeptide, α4β7",

author = "Claudia Fabrizio and Bavaro, {Davide F.} and Luigia Scudeller and Luciana Lepore and Flavia Balena and Antonella Lagioia and Gioacchino Angarano and L. Monno and Annalisa Saracino",

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doi = "10.1016/j.virol.2019.07.019",

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T1 - Variability OF HIV-1 V2 env domain for integrin binding

T2 - Clinical correlates

AU - Fabrizio, Claudia

AU - Bavaro, Davide F.

AU - Scudeller, Luigia

AU - Lepore, Luciana

AU - Balena, Flavia

AU - Lagioia, Antonella

AU - Angarano, Gioacchino

AU - Monno, L.

AU - Saracino, Annalisa

PY - 2019/9/1

Y1 - 2019/9/1

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