Variable reprogramming of the pluripotent stem cell marker Oct4 in mouse clones: Distinct developmental potentials in different culture environments

Michele Boiani, Luca Gentile, Vivian V. Gambles, Fatima Cavaleri, Carlo A. Redi, Hans R. Schöler

Research output: Contribution to journalArticle

Abstract

A prevailing view of cloning by somatic-cell nuclear transfer is that reprogramming of gene expression occurs during the first few hours after injection of the nucleus into an oocyte, that the process is stochastic, and that the type of reprogramming needed for cloning success is foreign and unlikely to be readily achieved in the ooplasm. Here, we present evidence that the release of reprogramming capacity is contingent on the culture environment of the clone while the contribution of aneuploidy to altered gene expression is marginal. In particular, the rate of blastocyst formation in clones and the regional distribution of mRNA for the pluripotent stem cell marker Oct4 in clonal blastocysts was highly dependent on the culture environment after cumulus cell nuclear transfer, unlike that in genetically equivalent zygotes. Epigenetic modifications of genetically identical somatic nuclei continue after the first cell division of the clones and are amenable to a degree of experimental control, and their development to the blastocyst stage and appropriate expression of Oct4 predict further outcome, such as derivation of embryonic stem (ES) cells, but not fetal development. This observation indicates that development to the blastocyst stage is not equivalent to full reprogramming and lends support to the novel concept that ES cells are not the equivalent of the inner cell mass, hence the discrepancy between ES cell derivability and fetal development of clones.

Original languageEnglish
Pages (from-to)1089-1104
Number of pages16
JournalStem Cells
Volume23
Issue number8
DOIs
Publication statusPublished - Sep 2005

Fingerprint

Pluripotent Stem Cells
Blastocyst
Embryonic Stem Cells
Clone Cells
Fetal Development
Organism Cloning
Stochastic Processes
Cumulus Cells
Gene Expression
Zygote
Aneuploidy
Epigenomics
Cell Division
Oocytes
Messenger RNA
Injections

Keywords

  • Embryo
  • Embryonic stem cell
  • Nuclear transfer
  • Oct4
  • Reprogramming

ASJC Scopus subject areas

  • Cell Biology

Cite this

Variable reprogramming of the pluripotent stem cell marker Oct4 in mouse clones : Distinct developmental potentials in different culture environments. / Boiani, Michele; Gentile, Luca; Gambles, Vivian V.; Cavaleri, Fatima; Redi, Carlo A.; Schöler, Hans R.

In: Stem Cells, Vol. 23, No. 8, 09.2005, p. 1089-1104.

Research output: Contribution to journalArticle

Boiani, Michele ; Gentile, Luca ; Gambles, Vivian V. ; Cavaleri, Fatima ; Redi, Carlo A. ; Schöler, Hans R. / Variable reprogramming of the pluripotent stem cell marker Oct4 in mouse clones : Distinct developmental potentials in different culture environments. In: Stem Cells. 2005 ; Vol. 23, No. 8. pp. 1089-1104.
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